山西地区TT病毒感染的分子流行病学研究  

The moleculer epidemiology of transfusion transmitted virus infection in Shanxi Province

作　　者：赵和平[1] 张玲荣[1] 杨慧珍[2] 张缭云[1] 郎丽娟[3] 

机构地区：[1]山西医科大学第一医院传染科,太原030001 [2]太原市商业职工医院妇产科 [3]山西医科大学第一医院传染科x,太原030001

出　　处：《中华实验和临床病毒学杂志》2000年第2期166-168,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的　了解山西地区不同人群中TT病毒 (TTV)感染状况及TTV山西分离株基因型分布情况。方法　用聚合酶链反应 (PCR)方法对山西地区不同人群血清标本进行检测 ,分析其基因型。结果　 2 4例慢性乙型肝炎患者、31例非甲～庚型肝炎患者、5 3例职业献血员、112例健康产妇中 ,TTVDNA阳性率分别为 41.6 6 %、2 9.0 3%、5 8.49%、8.93% ;18例 2～ 3岁健康儿童血清、10例TTVDNA阳性产妇的脐带血TTVDNA均阴性。 4株TTV山西分离株部分基因序列测定表明 ,同源性在 90 %以上 ,均属同一基因型。结论　本研究证实山西地区存在TTV感染 ,TTV感染可能是导致非甲非庚型肝炎的重要病原 ,TTV常重叠乙型肝炎病毒感染 ,而且在职业献血员和慢性肝病患者中存在很高的感染。该地区TTV病毒主要为 1a基因型。Objective To study the molecular epidemiology of transfusion transmited virus (TTV) infection and its distribution in different populations in Shanxi Province and the gene types of the isolates.Methods TTV DNA in serum samples was detected by polymerase chain reaction. Partial gene of the strains from different populations were cloned and sequenced, and their gene types were analyzed.Results Of 24 cases with chronic hepatitis B,31 cases with non A～G hepatitis, 53 cases of professional blood donors, 112 cases of healthy lying in women, the positive rates of TTV DNA were 41.66%,29.03%,58.49% and 8.93% respectively. TTV DNA in serum samples of 18 cases of 2～3 year old healthy children were negative and also were negative in umbilical cord blood of 10 cases of TTV DNA positive healthy lying in woman.Sequence analysis of 4 isolated strains of Shanxi Province showed that the nucleotide identity among them were more than 90% and they all belonged to the same gene type (la).Conclusions This study proved that TTV infection existed in Shanxi Province, TTV might be an important pathogen for non A to G hepatitis. TTV often co infeted with hepatitis B virus and there were high rates of TTV infection in professional blood donors and in paients with chronic liver disease. The gene type of TTV in Shanxi province was la.

关 键 词：聚合酶链反应 DNA病毒 序列分析 TTV病毒 

分 类 号：R181.3[医药卫生—流行病学]