中国和日本部分地区TT病毒的检出率及基因亚型分析  

High prevalence of TT virus (TTV) infection and gene subtype analysis between Japanese and Chinese

作　　者：刘卫东[1] 波田寿一 徐辉 程继东 东野一弥 

机构地区：[1]汕头大学医学院第二附属医院中心实验室,汕头515041 [2]日本国兵库医科大学第三内科

出　　处：《中华实验和临床病毒学杂志》2000年第2期169-172,共4页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的　比较中国及日本部分地区TT病毒 (TTV)感染情况及基因亚型分布特征。方法采用半巢式 聚合酶链反应 (Semi nestedPCR)检测中国和日本部分慢性丙型肝炎患者及正常人血标本中的TTV感染情况 ,并对阳性产物片段直接进行序列分析。结果　发现中国部分正常人群TTV感染率为 6 4% ;慢性丙型肝炎患者TTV感染率中国为 6 8% ,日本为 49.1%。基因亚型分布中国TTVG2a +2b亚型占 6 0 % ,G1a+1b亚型占 40 %。日本TTVG1a+1b亚型占 78.6 % ,G2a +2b亚型占 2 1.4%。结论　TTV在中日两国部分人群中均有较高的感染率 ,TTV基因亚型分布也有一定差异。Objective To compare the infection rates and gene subtypes of TTV between Japanese and Chinese.Methods TTV was detected by a semi nested PCR. The positive samples were sequenced directly.Results Infection rate of TTV in normal Chinese was 64%, but in the patients suffered from chronic C hepatitis, the infection rates were 68% in Chinese and 49.1% in Japanese. The gene subtype of TTV Gla+1b in Chinese was 40%, G2a+2b 60%; in Japanese TTV G1a+1b was 78.6%, G2a+2b 21.4%.Conclusion There were high infection rates of TTV in Chinese and Japanese. The gene subtype distribution of TTV had some difference between Chinese and Japanese.

关 键 词：肝炎病毒 TTV 聚合酶链反应 碱基序列 检出率 

分 类 号：R446.5[医药卫生—诊断学]