多维液相色谱分离技术在复杂蛋白质组学样品鉴定中的应用  

Application of Mudti-dimensional Liquid Chromatography in Complex Proteome Sample

作　　者：张罗敷[1] 胡晓芳[1] 徐学敏[1] 

机构地区：[1]上海交通大学,上海200030

出　　处：《现代生物医学进展》2013年第1期161-166,共6页Progress in Modern Biomedicine

基　　金：国家"973"课题(2010CB834300);国家自然科学基金项目(81071792)

摘　　要：目的:随着蛋白组学技术的发展,液相色谱-串联质谱的联用技术(液质联用)逐渐成为蛋白组学的主流技术。方法:通过结合各种不同原理的色谱分离类型,多维液相色谱分离技术能够极大的提高分离系统的峰容量,达到有效分离复杂程度很高的蛋白质组学样品的目的。结果:最广泛使用的多维液相色谱分离系统是离子交换色谱(IEX)和反相色谱(RP)的二维结合,近年来又发展出了分离能力更强的三维液相色谱分离系统,并且已经在蛋白质组学研究中得到了应用。结论:本文综述了多种多维液相色谱分离方法,在这些方法中,不同的分离原理的色谱类型被用于肽段或蛋白混合物的预分离中,有效促进了样品的充分分离,极大地提高了复杂样品的蛋白组学鉴定能力。Objective： Liquid chromatography - tandem mass spectrometry has been a mainstream technology in proteomics study. Method： Through the combination of various chromatography types, multi-dimensional liquid chromatography （MDLC） enables the great increase of peak capacity, realizing the effective separation of complex proteome sample. Result： Two-dimensional liquid chromatogra- phy （2DLC） with the combination of ion-exchange chromatography and reversed phase chromatography to separate peptides digested from complex samples is the most widely used MDLC approach. Recently, the addition of one-dimensional chromatography prior to the classic 2DLC to form three-dimensional liquid chromatography （3DLC） has been developed. Conclusion： Various MDLC methodologies are reviewed in this paper, in which different chromatography types have been exploited as additional first dimensional way to separate complex samples at peptide level or at protein level. The application 3DLC approaches promotes the more powerful resolution and in-depth identification of proteome samples.

关 键 词：蛋白质组 复杂样品 液质联用 二维液相色谱 三维液相色谱 

分 类 号：Q51[生物学—生物化学]