唐古特大黄多糖组分1对急性电离辐射损伤小鼠的保护作用  

Purified Fraction 1 of Polysaccharide Extracted from Rheum Tanguticum Prevents Acute Radiation-induced Damage in Mice

作　　者：刘琳娜[1] 郭志伟 张琰[1] 张甜[1] 

机构地区：[1]第四军医大学唐都医院药剂科,陕西西安710038 [2]新疆布尔津县人民医院,新疆布尔津836600

出　　处：《现代生物医学进展》2013年第2期213-215,248,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81073135)

摘　　要：目的:探讨唐古特大黄多糖组分1(RTP1)对急性电离辐射损伤小鼠的保护作用。方法:采用昆明种小鼠,随机分为5组:正常对照组(Normal Control,NC)、辐射对照组(Irradiation Control,IC)以及RTP1低剂量组(200 mg/kg)、中(400 mg/kg)和高剂量组(800 mg/kg),采用灌胃给药方式,连续14 d,NC组和IC组则给予等量的生理盐水,第14 d除NC组外,各组小鼠均接受2.0 Gy/只60Coγ射线照射1次,照射后24 h,检测小鼠胸腺和脾脏指数,测定肝脏超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性及丙二酰二醛(MDA)水平以及小鼠外周血象和骨髓嗜多染红细胞(PCE)微核数。结果:RTP1能够升高小鼠的胸腺、脾脏指数,增加肝脏SOD和GSH-Px活性,降低MDA水平,升高外周血中白细胞数并降低骨髓PCE微核数,与IC组比较有统计学意义(P<0.05或P<0.01)。结论:RTP1对辐射所致的小鼠损伤具有一定的保护作用。Objective： To investigate the protective effect of purified fraction 1 polysaccharide extracted from Rheum tanguticum （RTP1） on radiation-induced damage in mice. Methods： Kunming mice were randomly divided into five groups： normal group （NC）, irradiation control group （IC）, RTP1 low dose group （200mg/kg）, middle dose group （400mg/kg） and high dose group （800mg/kg）. The mice in RTP1 group were poured through gastic route by RTP1 for 14 d, the mice in NC and IC group were given 0.9% sodium chloride solution through the same way. The mice in all groups except NC group were irradiated with 2.0Gy 60Co γ-ray on the fourteenth day. The spleen and thymus index, the activity of SOD and GSH-Px, the content of MDA, the peripheral hemogram and the micronucleus rate of bone marrow polychromalic erythrocytes were detected at the 24th hour after radiation. Results： Compared with IC group, the spleen index, thymus index, the activity of SOD and GSH-Px, the number of WBC were increased markedly （P 〈 0.05 or P 〈 0.01）. The content of MDA and the micronucleus rates of bone marrow PCE were decreased significantly （P〈0.05 or P〈0.01）. Conclusion： RTP1 has an obvious protective effect on acute radiation induced mice injury.

关 键 词：唐古特大黄多糖 辐射损伤 60Coγ射线 辐射防护 

分 类 号：Q95-33[生物学—动物学] R285.5[医药卫生—中药学]