肾上腺素能β3受体基因多态性与冠心病及冠状动脉病变程度的相关性研究  

Relationship between β3-adrenergic receptor polymorphism and susceptibility to coronary heart disease and the degree of coronary artery stenosis

作　　者：崔国方[1] 王宁宁[1] 崔金兴[1] 

机构地区：[1]青岛市海慈医院心内科,山东省266033

出　　处：《中国心血管病研究》2013年第2期88-91,共4页Chinese Journal of Cardiovascular Research

摘　　要：目的探讨肾上腺素能β3受体（β3-AR）基因多态性与冠心病及冠状动脉病变的相关关系。方法选择冠心病患者120例，114名健康者为对照。应用聚合酶链反应-限制性片段长度多态性（PCR—RFLP）检测分析肾上腺素能良受体基因型，比较对照组与冠心病组的基因型及等位基因频率之间的差异，同时对冠心病患者中不同β3肾上腺素能受体基因型的冠状动脉病变程度进行比较。结果①冠心病组和对照组Trp64Trp、Trp64Arg和Arg64Arg的基因型频率分别为68．3％、30．0％、1．6％和69．2％、29．8％、0．9％。冠心病组Arg等位基因频率（16．6％）与对照组频率（15．8％）近似（P〉0．05），突变频率两组相比差异无统计学意义。②冠心病不同冠脉病变支数亚组间多态性分布差异无统计学意义（χ^2=0．471，P=0．790）。结论肾上腺素能艮受体基因多态性与冠心病及不同冠脉病变支数无相关性。Objective To investigate the association of β3-adrenergic receptor （β3-AR） gene polymorphisms of patients with coronary heart disease （CHD） and the degree of coronary artery stenosis. Methods The 120 patients with coronary heart disease and 114 healthy controls were involved in the study. These β3-adronergenil receptor genetic polymorphisms were analyzed by PCR-restriction fragment length polymorphism （PCR-PFLP）. The allele frequency distributions among case group and control group were analyzed. The relationship between genotype and the numbers of diseased vessds were analyzed of the patients with coronary heart disease. Results （1）The frequencies of Trp/Trp, Trp/Arg and Arg/Arg were 68.3%, 30.0%, 1.6% in the CHD group while those were 69.2%, 29.8% and 0.9% in the control group. The frequency of Arg allele were 16.6% in the CHD group and 15.8% in the control group （P〉0.05）. There was no significant difference between β3-AR Trp64Arg polymorphism with CHD and the control group. （2）The frequencies of Trp/Arg and Arg/Arg genotypes of the Trp64Arg polymorphism were not statistically difference among CHD patients with one, two and three or more significantly diseased vessels （χ2=0.471, P=0.790）. Conclusion The findings suggest no significant association was found between β3-AR Trp64Arg polymorphism and the extent of coronary artery stenosis among CHD patients.

关 键 词：冠状动脉疾病 肾上腺素能β3受体 基因多态性 冠状动脉病变 

分 类 号：R541.4[医药卫生—心血管疾病]