脑脊液CFP-10和Ag85检测对结核性脑膜炎的诊断价值  

Diagnostic value of cerebrospinal fluid CFP-10 and Ag85 detection on tuberculous meningitis

作　　者：王丹[1] 何玲[1] 初凤娜[1] 丁曼[1] 孙晓敏[1] 崔俐[1] 

机构地区：[1]吉林大学白求恩第一医院神经内科,长春130021

出　　处：《中国现代神经疾病杂志》2013年第2期121-125,共5页Chinese Journal of Contemporary Neurology and Neurosurgery

基　　金：吴阶平医学基金会临床科研专项基金资助项目~~

摘　　要：研究背景结核性脑膜炎的早期诊断目前仍是临床难点,寻找结核性脑膜炎早期特异性诊断指标是目前研究热点。本文主要探讨脑脊液培养分泌蛋白10(CFP10)和Ag85蛋白复合物表达水平对结核性脑膜炎的临床诊断价值。方法采用酶联免疫吸附试验分别检测结核性脑膜炎(30例)、非结核性颅内感染(27例)和对照(29例)受试者脑脊液CFP10和Ag85蛋白复合物表达水平。结果结核性脑膜炎组患者脑脊液CFP10和Ag85中位表达水平分别为0.74和1.10pg/ml,均高于非结核性颅内感染组[(0.02±0.01)、(0.54±0.10)pg/ml]和对照组[(0.02±0.01)、(0.52±0.11)pg/ml],组间差异有统计学意义(H=60.958,P=0.000;H=57.972,P=0.000)。非结核性颅内感染组与对照组之间差异无统计学意义(t=1.128,P=0.253;t=0.980,P=0.329)。结论结核性脑膜炎患者脑脊液CFP10和Ag85蛋白复合物表达水平高于非结核性颅内感染患者和对照者,检测这两项实验室指标的变化可协助结核性脑膜炎的早期诊断。Background Nowadays, the early diagnosis of tuberculous meningitis （TBM） is still one of the clinical difficulties, so the research focus is to seek out a specific marker of early TBM. This study is to evaluate the value of culture filtrate protein-10 （CFP-10） and Ag85 complex in cerebrospinal fluid （CSF） for the diagnosis of TBM. Methods According to the diagnostic criteria of TBM, 86 patients were divided into 3 groups： 30 patients with TBM; 27 with intracranial infection of non-TBM; 29 controls. CSF samples were collected by standard lumbar puncture and were used for detection of CFP-10 and Ag85 by enzyme-linked immunosorbent assay （ELISA）. Results The median concentrations of CFP-10 and Ag85 in CSF of the TBM group were 0.74 and 1.10 pg/ml. The average concentrations of CFP-10 and Ag85 in the intraeranial infection of non-TBM group and control group were （0.02 ＋ 0.01）, （0.54± 0.10） and （0.02 ± 0.01）, （0.52 ± 0.11） pg/ml respectively. The levels of CFP-10 and Ag85 in the TBM group were higher than that in the intracranial infection of non-TBM and the control group, and the differences were statistically significant （H = 60.958, P = 0.000; H = 57,972, P = 0.000）, but there was no significant difference bewteen the intracranial infection of non-TBM group and the control group （t = 1.128, P = 0.253; t = 0.980, P = 0.329）. Conclusion The levels of CFP-10 and Ag85 in the TBM group were significantly different from non-TBM group and control group. Detection of the two markers can be helpful in the early diagnosis of TBM.

关 键 词：结核 脑膜 脑脊髓液 细菌蛋白质类 酶联免疫吸附测定 

分 类 号：R521.7[医药卫生—内科学]