苏云金芽胞杆菌在小鼠肠道的消长及对小鼠肠道菌群的影响  

Dynamics of Bacillus thuringiensis in the intestinal tracts of mice and its effect on bacterial communities

作　　者：王小显[1] 杨洪凤[1] 薛雅蓉[1] 张明[2] 卜元卿[2] 刘常宏[1] 

机构地区：[1]南京大学医药生物技术国家重点实验室,江苏南京210093 [2]环境保护部南京环境科学研究所,江苏南京210042

出　　处：《畜牧与兽医》2013年第3期27-30,共4页Animal Husbandry & Veterinary Medicine

基　　金：国家环境保护部公益性行业科研专项(201009023)

摘　　要：利用选择性培养平板计数法,研究了苏云金芽胞杆菌(Bt)营养体和芽胞在小鼠肠道的数量消长及对小鼠肠道生理性有益菌群(大肠杆菌、双歧杆菌和乳酸杆菌)数量的影响,同时利用基于16S rDNA作为分子标记的变性梯度凝胶电泳(DGGE)研究了Bt对小鼠肠道微生物多样性的影响。结果表明:Bt营养体和芽胞在小鼠肠道内很快死亡,也未对小鼠肠道有益菌群数量和细菌多样性产生不良影响。说明苏云金芽胞杆菌(Bt)营养体及芽胞对小鼠是安全的。Dynamics of Bacillus thuringiensis （Bt） in the intestinal tract of mice and its effect on the population of the physiologically bene- ficial bacteria （Escherichia coli, Bifidobacterium spp. and Lactobacillus spp. ） was investigated using selective plate counting method. Mean- while, the effect of Bt on bacterial diversity in the intestinal tract was determined by denaturing gradient gel electrophoresis （DGGE） based on the 16S rDNA gene sequences. The results showed that the vegetative cells and spores of Bt could only survive at a short time in the intestine of mice and did not have adverse effect on the number of beneficial bacterial flora and diversity as well. It suggests that the vegetative cells and spores of Bt are safe for mice.

关 键 词：苏云金芽胞杆菌 肠道菌群 变性梯度凝胶电泳(DGGE) 

分 类 号：S852.6[农业科学—基础兽医学]