自发性高血压大鼠肝阳上亢证模型肾上腺组织的酪氨酸羟化酶基因表达研究  

STUDY ON ADRENAL MEDULLA TYROSINE HYDROXYLASE GENE EXPRESSION IN GAN YANG SHANG KANG SYNDROME MODEL OF SPONTANEOUS HYPERTENDION RATS

作　　者：鄢东红[1] 金益强[1] 唐发清[1] 肖纯[1] 胡随瑜[1] 王勇华[1] 张翔[1] 

机构地区：[1]湖南医科大学湘雅医院中西医结合研究所,长沙410008

出　　处：《中国现代医学杂志》2000年第8期24-26,共3页China Journal of Modern Medicine

基　　金：国家自然科学科学基本重点资助! (No3 9670 840 )

摘　　要：目的 :研究自发性高血压大鼠 (SHR)肝阳上亢证模型的酪氨酸羟化酶 (TH)基因表达水平及潜阳方对其影响。方法 :采用免疫组织化学方法和原位杂交法分别测定该模型肾上腺组织TH蛋白质及mRNA表达。结果 :SHR肝阳上亢证模型TH蛋白质及mRNA表达均明显增强 ,经用具有平肝熄风作用的潜阳方治疗后 ,其表达水平下降。提示 :SHR肝阳上亢证模型具有肾上腺组织TH表达增强的特点。Objective: To investigate the tyrosine hydroxylase (TH) gene expression in Gan Yang Shang Kang Syndrome (GYSKS) model of spontaneous hypertension rats (SHR) and the effects of Qian Yang Fang treatment. Methods: Adrenal medulla TH protein level was examined by immunohistochemistry and TH gene mRNA expression was determined by in situ hybridization. Results: TH protein level and mRNA expression in GYSKS model of SHR were obviously enhanced and with the treatment of Qian Yang Fang, the gene expression level decreased. Conclusions: Adrenal medulla TH gene expression enhanced is the character of GYSKS model of SHR.

关 键 词：高血压 肝阳上亢证 酪氨羟化酶 基因治疗 治疗 

分 类 号：R544.105[医药卫生—心血管疾病]