新生猪缺氧缺血性脑病模型肾血流动力学变化的研究  

Study on renal hemodynamics in newborn pigs model with hypoxic-ischemic encephalopathy

作　　者：乔伟[1] 任卫东[1] 肖杨杰[1] 张光华[1] 李红[2] 王晓明[2] 

机构地区：[1]中国医科大学附属盛京医院超声科,辽宁沈阳110004 [2]中国医科大学附属盛京医院放射科,辽宁沈阳110004

出　　处：《生物医学工程与临床》2013年第2期99-102,共4页Biomedical Engineering and Clinical Medicine

基　　金：辽宁省科技厅课题(2011225015);辽宁省教育厅课题(L2010659)

摘　　要：目的观察新生猪缺氧缺血性脑病模型肾动脉血流动力学改变及肾组织病理变化。方法 18只新生约克猪,雄性,猪龄3～5d,体质量2.0～2.5kg。随机分成对照组(n=8)和模型组(n=10)。模型组行气管插管、低氧通气及双侧颈动脉夹闭术,对照组不予低氧通气及双侧颈动脉夹闭。造模24h行肾脏超声检查,测量左、右肾动脉收缩期峰值血流速度(PSV)、舒张期末血流速度(EDV)及阻力指数(RI),取双侧平均值。对脑组织及肾组织行病理检测。结果与对照组相比,模型组PSV[(0.303±0.045)m/s vs(0.374±0.047)m/s]、EDV[(0.093±0.014)m/s vs(0.154±0.016)m/s]减低,RI(0.691±0.035 vs0.594±0.017)增高,差异有统计学意义(P<0.05)。模型组肾髓质部细胞肿胀、变性、坏死及凋亡;脑神经胶质细胞肿胀,神经元细胞变性及坏死。结论新生猪缺氧及脑缺血后早期除可直接引起脑损伤外,肾脏亦可出现低灌注,引起肾损伤。Objective To observe the changes in hemodynamics and pathology of damaged kidney of newborn pigs with hypoxie-ischemic encephalopathy（HIE）. Methods Eighteen male York pigs were enrolled, aged 3 - 5 days with body weight of 2.0 - 2.5 kg. All of them were randomly divided into the control group（n = 8） and the model group（n = 10）. The piglets were undergone bilateral carotid artery blockage and given 6 % oxygen mechanically in the model group, while only sham surgery was performed in the control group. After 24 hours, the average of peak systolic veloeity（PSV）, end diastolic velocity（EDV） and resistance index（R1） of left and right renal artery were measured by ultrasound. The pathological slices of myocardium and brain were observed. Results Compared with the control group, the value of PSV[（0.303 ± 0.045） m/s vs （0.374 ± 0.047） m/s] and EDV[（0.093 ± 0.014） m/s vs （0.154 ± 0.016） m/s] were reduced, and value of RI（0.691 ± 0.035 vs 0.594 ± 0.017） increased in the model group（P 〈 0.05）. In the model group, swelling, degeneration, necrosis and apoptosis were found in kidney medulla cells. Swelling, degeneration and necrosis were also found in some brain glial cells and neuronal cells in the model group. Conclusion It is demonstrated that newborn pig anaerobic and early cerebral ischemia could cause brain tissue damage, renal hypoperfusion, and renal tissue damage.

关 键 词：缺氧缺血 脑 猪 雏型 肾动脉 肾损害 

分 类 号：R722.1[医药卫生—儿科]