新生长白仔猪尾尖成纤维细胞的分离培养及其对猪瘟病毒的易感性分析  

Isolation of Landrace piglets tail-tip fibroblasts and its susceptibility to classical swine fever virus

作　　者：武蓉[1] 戴祯[1] 赵致阳[1] 高飞[1] 全龙泉[1] 逄大欣[1] 王铁东[1] 涂长春[2] 欧阳红生[1] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062 [2]军事医学科学院军事兽医研究所,吉林长春130062

出　　处：《中国兽医学报》2013年第3期326-329,共4页Chinese Journal of Veterinary Science

基　　金：转基因生物新品种培育重大专项资助项目(2011ZX08006-001)

摘　　要：以出生2日龄的正常长白仔猪尾尖组织为材料,通过组织消化法培养获得原代尾尖成纤维细胞。并通过间接免疫荧光法,梯度10倍倍比稀释猪瘟病毒石门株(105.2 TCID50/mL)感染在猪尾尖成纤维细胞,确定尾尖成纤维细胞最佳病毒感染滴度。结果显示,运用组织块消化法能够获得生长状态的原代猪尾尖成纤维细胞,并且细胞对10-3稀释倍数的猪瘟病毒石门株具有适中的感染力。In order to establish the mature pigtail-tip fibroblast cell isolation system and to evaluate its susceptibility to classical swine fever virus （CSFV） was Shimen strain, 2-day-old newborn Landrace pigs pigtail tip as the tissue material,and obtained primary fibroblasts of the pig tail-tip by organization digestion method, then used indirect immunofluorescence method to determine the fibroblasts susceptibility to the virus（105.2 TCID50/mL） in a gradient dilution testing. Our results showed that organization digestion method can able to obtain pig tail tip fibroblast cells grow in good condition and it is proper susceptibility to CSFV Shimen strain with 10.3 fold dilution of vi- rus stock solution.

关 键 词：猪尾成纤维细胞 分离 细胞培养 转基因动物 病毒易感性猪 

分 类 号：S852.65[农业科学—基础兽医学]