试验性脂肪肝出血综合征鸡氧化应激水平和内皮功能变化  

Oxidative stress and endothelial function in chickens with experimental fatty liver hemorrhagic syndrome

作　　者：姜锦鹏[1] 顾有方[1] 吕锦芳[1] 宁康健[1] 应如海[1] 胡忠泽[1] 李静[1] 

机构地区：[1]安徽科技学院动物科学学院,安徽凤阳233100

出　　处：《中国兽医学报》2013年第3期439-444,共6页Chinese Journal of Veterinary Science

基　　金：安徽省自然科学基金资助项目(11040606M88);安徽科技学院兽医学重点学科基金资助项目(AKXK20101-2);安徽科技学院进校人才基金资助项目(ZRC2008206)

摘　　要：将320只14日龄青脚麻鸡随机分为对照组、高脂模型组、雌激素模型组和高脂结合雌激素模型组,每组设4个重复,每个重复20只,共处理28d。造模14、28d,测定肝脏氧化应激、血清肝功能和内皮功能相关参数,并进行肝脏病理组织学观察。结果显示,模型组鸡肝脂含量增加、肝细胞脂肪变性,肝脏血管损伤,出现典型的鸡脂肪肝出血综合征(FLHS)病变;与对照组相比,各模型组肝脏MDA含量升高(P<0.05),SOD和GSH-Px活性降低,血浆AngⅡ含量增加,血清NO含量降低;相关性分析显示,肝脏脂肪变性程度与肝脏MDA含量正相关(P<0.01),与肝脏SOD和GSH-Px活性呈负相关(P<0.01);肝脏MDA含量与肝出血分数和血浆AngⅡ含量皆有极显著的相关性(P<0.01);肝脏SOD和GSH-Px活性与肝出血分数呈负相关(P<0.01或P<0.05),与血清NO含量的变化均呈正相关(P<0.05);血浆AngⅡ含量与肝出血分数呈正相关(P<0.05)。结果表明,FLHS发生和发展过程中氧化应激参与了内皮功能紊乱的形成,血管损伤可能与氧化应激和内皮功能失调有关。The present study aimed to explore the relationship between oxidative stress and endo- thelial function in chickens with fatty liver hemorrhagic syndrome （FLHS）. Three hundred twen- ty 14 day-old cyan-shank partridge chickens were divided randomly to 4 treatment groups with 4 replications of 20 each,including control （C）, high-fat diet （HF）, estrogen-injected （E）, high fat diet plus estrogen-injected （HF＋E）. The experimental period lasted from 15 to 42 day-old. He- patic histopathology and biochemical parameters related to liver oxidative stress, liver function and endothelial function were determined at 14 d interval. The results showed as following： （1） the chickens in model groups displayed severe impairment of liver histology （increased liver total lipids, hepatic steatosis and liver hemorrhage）, reproducing typical histological features of chicken FLHS; （2） compared with the control group,the contents of malondialdehyde （MDA） （P〈0.05） in liver and angiotensin Ⅱ （Ang Ⅱ ） in plasma were increased, whereas the activity of superoxide dismutase （SOD） and glutathione peroxidase （GSH-Px） in liver and the contents of serum nitric oxide （NO） were decreased in model groups on day 14 and 28;（3） hepatic steatosis was positively correlated with MDA （r=0. 949,P〈0.01）,and negatively correlated with SOD （r=-0. 864,P 〈0.01） and GSH-Px （r= -0. 897,P〈0.01）. MDA was positively correlated with liver hemor- rhage score （r=0.859,P%0.01） and plasma AngⅡ （r=0.902,P〈0.01）.Activity of SOD or GSH-Px in liver was inversely correlated with liver hemorrhage score （r=-0. 901 ,P〈0.01 or r=-0. 760,P〈0.05） and positively correlated with NO （r=0. 714,P〈0.05 or r=0. 734,P〈 0.05）. Plasma levels of Ang Ⅱ were positively correlated with liver hemorrhage score （r= 0. 784, P d0.05）. The trial shows that excessive oxidative stress is involved, at least in part,in endothelial dysfunction and the mechanism of vascular injury may be

关 键 词：脂肪肝出血综合征 鸡 氧化应激 内皮功能 

分 类 号：S831.7[农业科学—畜牧学]