H_2O_2诱导BRL-3A细胞模拟大鼠肝脏氧化应激损伤的研究  

Simulating oxidative stress damage of rat liver through H_2O_2 induction on BRL-3A cells

作　　者：臧琳[1] 郭景茹[2] 郭爽[1] 王慧[1] 计红[1] 甄莉[1] 汪志 杨焕民[1] 

机构地区：[1]黑龙江八一农垦大学动物科技学院,黑龙江大庆163319 [2]吉林大学畜牧兽医学院,吉林长春130062 [3]天津嘉立荷牧业有限公司,天津300402

出　　处：《中国兽医学报》2013年第3期445-449,共5页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(30972159);黑龙江省2011年研究生创新科研基金资助项目(YJSCX2011-274HLJ);黑龙江省教育厅面上项目(12521371)

摘　　要：动物冷暴露过程中,肝脏因发生氧化应激而受损。利用不同浓度过氧化氢(H2O2)作用BRL-3A细胞,根据细胞存活率确定500μmol/L H2O2作用细胞3h为施加条件,然后通过检测蛋白质羰基含量、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和过氧化氢酶(CAT)活性,探讨在细胞水平上建立冷暴露大鼠肝脏损伤模型。结果显示,H2O2处理组细胞存活率下降,蛋白质羰基含量升高,SOD、GSH-Px和CAT活性均降低。与对照组相比,其存活率、细胞内蛋白质羰基含量和CAT活性,均差异极显著(P<0.01),细胞内SOD和GSH-Px活性,差异显著(P<0.05)。结果表明,500μmol/L H2O2作用于BRL-3A细胞可导致氧化应激的发生,其相关指标与大鼠冷暴露后肝脏受损指标改变趋势类似,利用BRL-3A细胞建立冷暴露引起的肝脏氧化损伤模型,为进一步探讨冷应激对肝脏损伤的机制奠定基础。The animal liver can be damaged by oxidative stress during cold exposure. In this study, different doses of hydrogen peroxide （H2O2） were used to treat BRL-3A cells,and the optimal concentration was then chosen by measuring the cell survival rate. In this way, we selected the 500 μmol/L H2O2 as an induced concentration acting on BRL-3A cells for 3 h,then tried to establish an experimental BRL-3A cell model of liver damages in rat simulating cold exposure using H2 02, to detect the protein carbonyl content, and the activities of superoxidase dismutase（SOD）,gluta- thione peroxidase （GSH-Px） and catalse（CAT）. Results showed that in the H2 O2-treated group, a decrease in cell survival rate, an increase in protein carbonyl content and a decrease in SOD, GSH-Px and CAT activities were observed. And comparing to the control group, a highly signifi- cant difference （P〈0.01） was found in cell survival rate,protein carbonyl content and CAT ac- tivity,and a rather significant difference （P〈0.05） in SOD and GSH-Px activities. Thus,the re- sults proved that an oxidative stress could be generated when H2O2 actied on BRL-3A cells,and the change trends of indicators were similar to those of a real cold-exposed-and-liver damage in rat. Thereby, this study makes it possible to establish a cell model of stimulating hepatic oxidative damage by cold ex- posure using H2 02 induction on BRL-3A ceils, which will set up a solid base for the future studies on theother relative mechanisms on cold stress.

关 键 词：过氧化氢 BRL-3A细胞 冷暴露 肝脏 氧化应激 

分 类 号：S852.2[农业科学—基础兽医学]