辽宁绒山羊皮肤毛囊VEGF基因表达的RT-PCR检测  

Detection of VEGF gene expression in hair folliclesof liaoning cashmere goats by RT-PCR

作　　者：姚纪元[1] 李玉梅[2] 徐海录[3] 杨金生[4] 姜怀志[1] 

机构地区：[1]吉林农业大学动物科学技术学院,吉林长春130118 [2]吉林大学畜牧兽医学院,吉林长春130062 [3]东丰县动物疫病预防控制中心,吉林东丰136300 [4]吉林省畜牧兽医科学研究院,吉林长春130062

出　　处：《中国兽医学报》2013年第3期477-480,共4页Chinese Journal of Veterinary Science

基　　金：国家十一五科技支撑计划资助项目(2009BADA5B03)

摘　　要：从辽宁绒山羊常年长绒和季节长绒2个品系皮肤毛囊组织中提取总RNA,以总RNA为模板进行RT-PCR扩增血管内皮生长因子(VEGF)基因并进行相对定量表达研究。结果显示,VEGF基因mRNA在辽宁绒山羊常年长绒和季节长绒2个品系的皮肤毛囊组织中均有表达,并且2个品系在1年内同一月份VEGF基因mRNA的相对表达量差异不显著(P>0.05),2月份VEGF基因mRNA的相对表达量与其他月份差异显著(P<0.05)。结果表明,常年长绒型新品系VEGF基因mRNA的相对表达量除2月外,其他月份均常年维持在一个较高的水平。Total RNA al plush two strains, method and relative was isolated from the then the VEGF gene expression was also pressed in both of these two strains, and cashmere goats skin of the perennial plush and season- was amplified with the RNA as template by RT-PCR determined. The result showed that VEGF was ex- the differential expression between them in the same month within one year was not significant （P〉0.05）. But the expression level of the VEGF in the February was highest compared to that of the other month （P〈0.05）, and the expression of that in the perennial plush strain was maintained a highly level except for that in the February.

关 键 词：VEGF 毛囊 RT-PCR 辽宁绒山羊 

分 类 号：S852.2[农业科学—基础兽医学]