聚乙烯亚胺包被的四氧化三铁磁性纳米颗粒对SHI-1细胞生物学特性的影响  

Biocompatibility of polyethylene imine （PEI）-coated magnetic Fe3O4 nanoparticles in SHI-1 cells

作　　者：陈晓丽[1] 陈子兴[1] 陈苏宁[1] 岑建农[1] 祁小飞[1] 姚利[1] 王元元[1] 

机构地区：[1]苏州大学附属第一医院、江苏省血液研究所,215006

出　　处：《中华血液学杂志》2013年第3期185-189,共5页Chinese Journal of Hematology

基　　金：科技部重大科研“973”计划（2011CB933501）

摘　　要：目的初步探讨利用聚乙烯亚胺包被的四氧化三铁（PEI—Fe3O4）磁性纳米颗粒进行磁共振细胞影像技术跟踪细胞生物学行为的可行性。方法以人急性单核细胞白血病细胞株SHI-1为对象，用透射电镜观察PEI-Fe3O4磁性纳米颗粒能否被细胞内吞；用电感耦合等离子发射光谱仪及普鲁士蓝染色观察影响细胞磁性纳米颗粒载量的因素；用CCK-8法检测PEI—Fe3O4对SHI-1细胞增殖的影响；用流式细胞术（FCM）检测PEI—Fe3O4磁性纳米颗粒对细胞分化的影响；以甲基纤维素半固体培养基培养法检测PEI-Fe3O4对细胞集落形成能力的影响。以未经PEI—Fe3O4磁性纳米颗粒处理的SHI-1细胞作为对照组。结果PEI-Fe3O4磁性纳米颗粒能成功被SHI-1细胞内吞，细胞内磁性纳米颗粒载量与其起始浓度（以纳米颗粒中的铁离子浓度代表PEI—Fe3O4浓度，即p,gFe／ml）及与细胞共培养的时间呈正相关，随细胞分裂传代而减少。以5～100μgFe／mlPEI-Fe3O4处理细胞，60～100μgFe／ml各组对细胞增殖抑制率高于5～50μgFe／ml各组，差异具有统计学意义（P〈0．05）；FCM检测对照组细胞CD11b和CD14分子的表达分别为（78．4±18．5）％和（18．7±2．9）％，佛波醇酯（TPA）处理后增至（92．1±6．5）％和（20．8±2．3）％，PEI-Fe3O4标记后的细胞CD11b和CD14分子的表达分别为（83．3±14．2）％和（20．4±2．1）％，TPA处理后增至（95．8±3．3）％、（21．0±6．9）％，实验组和对照组间在TPA处理前后的CD11b和CD14表达差异无统计学意义（P〉0．05）；0、20、50μgFe／mlPEI—Fe304处理24h后的SHI-1细胞集落形成率分别为（25．20±7．22）％、（25．93±13．15）％和（23．37±9．33）％，各组间差异无统计学意义（P〉0．05）。结论PEI—Fe3O4磁性纳米颗粒可高效率标记SHI-1细胞，在5—50μg Fe／ml范围内，与SHI-1细胞具有很好的生物相容性，不影响Objective To explore the feasibility of magnetic resonance cell imaging technology by using polyethylene imine （PEI）-coated magnetic nanoparticles of Fe3O4 （PEI-Fe3 O4-MNPs） to track cell bi- ology behavior. Methods Endocytic PEI-Fe3O4-MNPs in SHI-1 cells were observed by transmission electron microscopy （TEM） . Iron contents of nano-labeled cells were analyzed by inductively coupled plasma-atomic emission spectroscopy （ICP-AES） and Prussian blue staining. The proliferation ability of labeled cells was detected by cell counting kit-8 （CCK-8） assay; the differentiation and colony-forming abilities were also ob- served. SHI-1 cells without endocytosing PEI-Fe3O4-MNPs were used as control. Results Our data showed that PEI-Fe3O4-MNPs could label SHI-1 cells. The labeling efficiency depended on the nanoparticles＇ con- centration and the duration of cells treating. Inhibition rates of SHI-1 cells labeled by 60 -100 μg Fe/ml PEI- Fe3O4-MNPs were much higher than of 5 -50μg Fe/ml ones following treating by 5 - 100 μg Fe/ml PEI- Fe3 O4-MNPs for 48 hrs. The expressions of CD11 b and CD14 were （78.4 ± 18.5 ） % and （ 18.7± 2.9） % in control vs （83.3 ＋ 14.2）% and （20.4 ± 2.1 ）% in cells fractions treated by 30 μg Fe/ml PEI-Fe3O4-MNPs. Clony-forming rates of SHI-1 cells labeled by 0, 20 , 50 μg Fe/ml PEI-Fe3 04-MNPs were （25.20 ± 7.22） %, （25.93 ± 13.15 ） %, （23.37 ± 9.33 ） %, respectively. Differentiation and colony-forming poten- tials of labeled cells were similar with control in the certain range of PEI-Fe3O4-MNPs concentration. Conclusion SHI-I cells were efficiently labeled by PEI-F%O4-MNPs with well biocompatibilities in proper range of concentration, the latter could be coupled with magnetic resonance imaging （MRI） to track cells in vivo.

关 键 词：白血病 磁性纳米颗粒 四氧化三铁 聚乙烯亚胺 生物相容性 

分 类 号：R7[医药卫生—临床医学] TB38[一般工业技术—材料科学与工程]