两种甲型肝炎病毒抗原快速检测方法的建立  

Development of two rapid detection methods for hepatitis A virus

作　　者：龙润乡[1] 杨蓉[1] 李华[1] 杨婷[1] 罗芳宇 王俊[1] 谢忠平[1] 

机构地区：[1]中国医学科学院北京协和医学院医学生物学研究所云南省重大传染病疫苗工程技术研究中心,昆明650118

出　　处：《微生物学免疫学进展》2013年第1期39-41,共3页Progress In Microbiology and Immunology

基　　金：科技部国家支撑计划项目;项目编号2008BAI66B04;中国医学科学院医学生物学研究所项目;项目编号:2006IMB05

摘　　要：目的建立两种甲型肝炎病毒抗原(HAV-Ag)检测试剂盒,并对其检测效果进行评价。方法生物素标记甲型肝炎病毒抗体(HAV-Ab)与辣根过氧化物酶标记亲和素联合应用建立甲型肝炎病毒抗原BA-ELISA检测法;同时使用辣根过氧化物酶标记HAV-Ab作放大系统建立双抗体夹心甲型肝炎病毒抗原ELISA检测试剂,对比两种检测方法的特异性、灵敏度及实际应用效果。结果用生物素标记甲型肝炎病毒抗体-辣根过氧化物酶标记亲和素作放大系统建立的甲型肝炎病毒抗原BA-ELISA检测法,较双抗体夹心ELISA检测方法灵敏度高1～2个稀释度;两种检测法均对10余种病毒无交叉,P/N值BA-ELISA检测法较高。结论甲型肝炎病毒抗原BA-ELISA检测法是一种灵敏度高,特异性好,方便快捷的检测方法,可广泛应用于甲型肝炎病毒研究及临床检测中。而甲型肝炎病毒抗原双抗体夹心ELISA检测法,检测灵敏度适中,操作简单,更适用于甲肝疫苗生产检定。Objective To establish two kinds of rapid detection methods for hepatitis A virus antigen （HAV-Ag） and evaluate the detection effect. Methods Hepatitis A virus antigen BA-ELISA assay was established by biotin-labeled hepatitis A virus antibody （HAV-Ab） in combination with avidin-labeled HRP. Double antibody sandwich method of ELISA assay was established using hepatitis A virus HRP-labeled antibody. Two kinds of detection methods were compared for their specificity, sensitivity and the practical application effect. Results The sensitivity of BA-ELISA detection method is higher 1-2 dilution then that of double antibody sandwich detection method. Two kinds of detection methods have not cross reaction with 10 types of virus. P/N value of BA-ELISA assay is higher. Conclusion The hepatitis A virus antigen BA-ELISA assay is a method that has the advantages of convenient, rapid detection, and can be widely applied to hepatitis A virus research and clinical testing. Double antibody sandwich ELISA assay has moderate detection sensitivity, the operation is simple, and more suitable for hepatitis A vaccine production test.

关 键 词：甲型肝炎病毒 酶联免疫检测 生物素-亲和素系统 

分 类 号：R446.6[医药卫生—诊断学]