粤黄鸡IL-15基因真核表达及其多克隆抗体的制备  

Eukaryotic Expression of Yuehuang Chicken Interleukin 15 Gene and Preparation of Its Polyclonal Antibody

作　　者：尹会方[1,2] 黄青云[1] 王家玲[1] 伍世雄[1] 叶日松[1] 

机构地区：[1]华南农业大学兽医学院,广东广州510642 [2]福建龙岩学院生命科学学院,福建龙岩364000

出　　处：《中国家禽》2013年第5期18-21,共4页China Poultry

基　　金：广东省自然科学基金(20043227)

摘　　要：本研究参照GenBank登录的鸡白细胞介素15(ChIL-15)基因序列自行设计一对特异性引物,以RT-PCR、基因克隆,重组真核表达质粒构建、表达及试验兔免疫等技术,成功获得粤黄鸡IL-15基因:全长564bp,编码187个氨基酸,与GenBank上已发表的其它品种鸡IL-15基因序列及其推导的氨基酸序列相似性为99.1%～100%;成功构建鸡IL-15基因重组真核表达质粒pcDNA-ChIL-15;成功制备兔抗鸡IL-15多克隆抗体。为进一步研究粤黄鸡IL-15的生物学功能,试验pcDNA-ChIL-15作鸡的各种新型DNA疫苗的分子免疫佐剂等奠定基础。A pair of specific primers was designed according to the published sequences of interleukin 15 （IL-15） of chicken in GenBank. Interleukin 15 gene was amplified by RT-PCR from splenic lymphocytes of Yuehuang chicken, the IL-15 gene of Yuehuang chicken was 564 bp in length, encoding 187 amino acids. The amino acid homologies were 99.1% to 100% compared with the the reported IL-15 of chicken. The gene was then cloned into the expression vector pcDNA3.1 to construct plasmid pcDNA-ChlL-15. And polyclonal antibody was gained after immunizing rabbits with pcDNA-ChIL-15. These findings will be of benefit to further study the role of IL-15 and discuss immune adjuvant effect of pcDNA-ChIL-15 as a novel DNA vaccine immune adjuvant of chicken.

关 键 词：鸡IL-15基因 真核表达 多克隆抗体 

分 类 号：S852.659.6[农业科学—基础兽医学]