细胞周期蛋白依赖性激酶10在瘢痕疙瘩中的表达及其临床意义  

The expression of cyclin-dependent kinase 10 in keloid and its clinical significance

作　　者：杨琳[1] 杨大平[1] 肖志波[1] 刘莺[1] 

机构地区：[1]哈尔滨医科大学附属第二医院整形美容科,黑龙江哈尔滨150086

出　　处：《中国美容整形外科杂志》2013年第3期148-151,共4页Chinese Journal of Aesthetic and Plastic Surgery

摘　　要：目的研究细胞周期蛋白依赖性激酶10在瘢痕疙瘩中的异常表达及其在瘢痕疙瘩发生机制中的作用。方法应用反转录聚合酶链式反应（1it．PCR）及Western Blot检测细胞周期蛋白依赖性激酶10mRNA及细胞周期蛋白依赖陛激酶10蛋白在人正常皮肤成纤维细胞及人瘢痕疙瘩成纤维细胞中的表达；将外源重组性真核质粒pCMV6-细胞周期蛋白依赖性激酶10转染到人瘢痕疙瘩成纤维细胞内为实验组；转染空质粒细胞及未处理细胞为对照组。用MTF法检测瘢痕疙瘩成纤维细胞增殖状况。结果与正常皮肤成纤维细胞相比，细胞周期蛋白依赖陛激酶10mRNA和细胞周期蛋白依赖f生激酶10蛋白在瘢痕疙瘩成纤维细胞中的表达显著下调，差异具有统计学意义（P〈0．01）；与空pCMV6组和未转染组相比，pCMV6-细胞周期蛋白依赖性激酶10组细胞生长明显受到抑制，差异具有统计学意义（P〈0．01）。结论CDKl0在瘢痕疙瘩中呈下调表达，以CDKl0为靶点的基因治疗有潜在的临床应用前景。Objective To investigate the abnormal expression of cyclin-dependent kinase 10 in Keloid and the roles in mechanism of the keloid production pathogeny. Methods The expression of cyclin-dependent kinase 10 mRNA and protein in normal skin fibrobtast and Keloid fibroblast was measured by reverse transcription- poly- merase chain reaction （RT-PCR） and Western Blot, respectively. A recombinant plasmid carrying the cyclin-de- pendent kinase 10 gene （pCMV6-cyclin-dependent kinase 10） was transfected into keloid fibroblast as the experi- mental group, and keloid fibroblast untransfected and those transfected with empty pCMV6 plasmid were used as the control group. MTI＂ was applied to measure the effect of cyclin-dependent kinase 10 on the proliferation of Ke- loid fibroblasts. Results Compared with normal skin fibroblast, the expression of cyclin-dependent kinase 10 mRNA and protein was down-regulated in Keloid fibroblast （ P 〈 0.01 ）. The growth of keloid fibmblast in the pCMV6-cyclin-dependent kinase 10 group was significantly lower than that in the empty pCMV6 and untransfeeted groups（ P 〈 0.01 ）. Conclusion cyclin-dependent kinase 10 is down-regulated in Keloid fibroblast compared with normal skin fibroblast. The CDK10 gene is a potential target for gene therapy of Keloid.

关 键 词：细胞周期蛋白依赖性激酶10 瘢痕疙瘩 反转录聚合酶链式反应 蛋白质印迹 

分 类 号：R619.6[医药卫生—外科学]