强直性脊柱炎棘上韧带组织病理学和超微结构观察  

Histopathologicai and ultrastructural observation of the supraspinal ligaments in ankylosing spondylitis

作　　者：许红飞[1] 张乐 张莹[1] 初同伟[1] 

机构地区：[1]第三军医大学新桥医院骨科,重庆400037 [2]中心实验室

出　　处：《中华风湿病学杂志》2013年第3期152-154,F0003,共4页Chinese Journal of Rheumatology

基　　金：国家自然科学基金（81071523）;重庆市自然科学基金（CSTC2011BA5009）

摘　　要：目的观察强直性脊柱炎（As）棘上韧带在脊柱骨性强直后发生的组织病理学及超微结构改变。方法通过光镜下观察苏木素．伊红（HE）、苦味酸一天狼星红染色，并行电镜观察微观结构改变，与对照组进行比较。图像处理软件分析血管密度、胶原原纤维分布密度。采用t检验进行统计学分析。结果实验组光镜下，胶原纤维排列紊乱，可见玻璃样变及脂肪浸润，大量血管增生，管径不一，有Ⅲ型胶原表达，电镜下胶原纤维分布稀疏，周期性横纹模糊，基质降解，可见基质小泡。对照组胶原纤维排列规则，无上述改变。实验组血管密度（6．8±0．7）／HP，对照组（1．0±0．4）／HP；实验组胶原原纤维分布密度（122±22）／μm2，对照组（218±10）／μm2，2组差异有统计学意义（P〈O．05）。结论棘上韧带在AS疾病进展过程中出现一系列的组织病理学改变。Objective To observe the histopathological and ultrastructnral changes of the supraspinal ligaments in ankylosing spondylitis （AS）. Methods The supraspinal ligaments were stained with HE, Sirius Red/saturated carbazotic acid and then observed nuder microscope, electyonic scanning microscope and transmission electron microscope respectively. The density of blood vessel and collagen fibril were analy-zed. Statistical analysis was carried out by using t test. Results In the AS group, under the microscope, the arrangement of in collagen fibers was in chaos, and hyalinosis, fatty infiltration could be seen. Vascular proli- feration was predominant. Collagen-lU expression could be detected in this group. Under electron microscope, the distribution of collagen fibril was sparse, and the periodic strita was indistinct, the matrix was degraded, and some matrix vesicle could be seen. While in the control group, orderly arrangd collagen fibrils could be seen, and no change of blood vessels could be seen. The density of vascular structures in the AS group was （6.8±0.7）/HP, and （1.0±0.4）/HP of the control group. The density of collagen fibrils in the AS group was （122±22）/μm2, and that of the control group was （218±10）/μm2, the difference between the two groups was statistically significant （P〈0.05）. Conclusion A series of histopathological changes have occurred in the supraspinal ligaments in AS patients.

关 键 词：强直性 脊柱炎 棘上韧带 组织病理学 超微结构 

分 类 号：R593.23[医药卫生—内科学]