两株H6N8亚型禽流感病毒分离株的分离与鉴定  

Sequence analysis and identification of 2 H6N8 subtype avain influenza virus isolates

作　　者：张曦[1] 崔鹏飞[1] 朱占松[1] 谭丹[1] 李文辉[1] 关立峥[1] 邓国华[1] 陈化兰[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所农业部动物流感重点开放实验室/兽医生物技术国家重点实验室,黑龙江哈尔滨150001

出　　处：《中国预防兽医学报》2013年第3期177-180,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家973基金项目(2011CB505001)

摘　　要：为了解禽流感病毒(AIV)在我国华南地区的流行情况,我们对活禽市场中分离得到的两株鸭源H6N8亚型AIV[A/duck/Fujian/S2191/2011(H6N8)(FJ/191/11)和A/duck/Guizhou/S4035/2011(H6N8)(GZ/035/11)]进行全基因序列测定,并进行其对SPF鸡和BALB/c小鼠的致病性试验。序列分析显示:HA裂解位点基序为339PQIETR↓GLFG348,为低致病性AIV特征。内部基因分别源于两个国内流行的H6亚型病毒家系(ST339-like和HuN573-like)。序列分析表明FJ/191/11和GZ/035/11为H6亚型病毒与其他亚型AIV的N8NA基因发生重组,呈现明显的异源性。两分离株的感染性试验显示,它们对鸡和小鼠均呈低致病性;而且不能在鸡体内有效复制;对小鼠的感染性实验结果显示,小鼠肺脏和鼻甲病毒分离结果为阳性,其他脏器病毒滴定结果为阴性。To investigate the prevalence of avian influenza virus （A1V） in Southem China, two AIVs （FJ/191/11and GZ/035/11） of H6N8 subtype were isolated from live-poultry markets during 2011. The sequencing results shows that both isolates had a HA cleavage site of ^339pQIETR↓ GLFG^348, which is a characteristic of low pathogenesis AIV. The phylogenetic analysis showed that the internal genes of the two isolates originated from two major virus lineages of H6 subtypes in China （ST339-1ike and HuN573-1ike）. Experimental infection tests showed that the isolates were low-pathogenic for mouse which were only replicated in nasal shall and lung, and caused a slight histopathogical changes. However, the isloates were low pahogenic in chickens.

关 键 词：禽流感病毒 H6N8亚型 抗原分析 致病性 

分 类 号：S852.65[农业科学—基础兽医学]