我国东北地区野生鸟类A亚群禽白血病病毒分子流行病学调查及env基因序列分析  

Molecular epidemiology and env gene sequence analysis of avian leukosis virus isolated from wild birds in China

作　　者：杨波[1,2] 高玉龙[2] 高宏雷[2] 秦立廷[2] 刘婉思[1,2] 李德龙[3] 高奇[2] 曾祥伟[1] 王笑梅[2] 

机构地区：[1]东北林业大学野生动物资源学院,黑龙江哈尔滨150040 [2]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/禽传染病研究室,黑龙江哈尔滨150001 [3]山东农业大学动物科技学院,山东泰安271000

出　　处：《中国预防兽医学报》2013年第3期245-247,共3页Chinese Journal of Preventive Veterinary Medicine

基　　金：中央高校基本科研业务费专项资金(DL12CA14);中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室开放基金项目(SKLVBF201201);黑龙江省自然科学基金项目(C201223);国家林业局野生动物疫源疫病监测项目(2012);公益性行业(农业)科研专项经费(201203055)

摘　　要：为了解野生鸟类禽白血病病毒(ALV)的感染情况,本研究采集了300份野生鸟类样品,将样品处理后接种DF-1细胞,利用p27抗原ELISA、IFA、PCR等方法检测,其中两份样品为ALV阳性并对其env基因扩增。结果表明,其中gp85编码序列与已发表的A亚群ALV(ALV-A)的同源性最高,为91.1%～100%,而与已发表的鸡的B、C、D、E、J亚群ALV的gp85编码序列的同源性仅在28.0%～80.3%之间。遗传进化树分析也表明这两份ALV阳性样品的gp85编码序列属于ALV-A。本实验在我国野生鸟类群体中首次分离和鉴定出ALV-A,表明目前我国野生鸟类已经存在A亚群ALV的感染。To investigate the situation of wild birds infected avian leukosis virus （ALV） in Northeast China, we collected 300 samples of wild birds fi＇om different areas, and 2 samples were ALV positive detection by p27 ELISA, indirect immunofluorescent antibody assay and PCR. The ALVs were further identified as ALV subgroup A by env gene sequencing analysis. Furthermore, the gp85 deduced amino acid sequence analysis indicated that the two isolates had the highest homology with reference strains of subgroup A in the range of 91.1% to 100%, but the homology was only in the range of 28.0% to 80.3% with other subgroups B, C, D, E and J. To the best of our knowledge, this is the first report on isolation and identification of ALV subgroup A in wild birds.

关 键 词：野生鸟类 A亚群禽白血病 gp85 序列分析 

分 类 号：S852.65[农业科学—基础兽医学]