赋值传递与方法学比对对提高糖化血红蛋白可比性的观察  

Improvement in the comparability of HbA1c by transferring value-assignment and methodology comparison

作　　者：俞叶夫[1] 包国祥[2] 赵国民 杨贵儿[4] 

机构地区：[1]绍兴文理学院附属医院检验科,312000 [2]绍兴市人民医院检验科 [3]绍兴市富盛镇社区卫生服务中心检验科 [4]绍兴文理学院附属医院内科,312000

出　　处：《中国糖尿病杂志》2013年第3期264-266,共3页Chinese Journal of Diabetes

摘　　要：目的提高不同检测系统测定HbA1c结果的可比性,为实验室间HbA1c结果的互认提供参考。方法用新鲜混合全血标本赋值传递并行方法学比对,比较赋值传递前后胶乳增强免疫比浊法(LETIA)与高效液相色谱法(HPLC)的可比性。结果与HPLC法相比LETIA法经赋值传递后在医学决定水平处的偏倚由8.83%下降到0.65%。结论用新鲜混合全血标本作赋值传递并行方法学比对可提高HbA1c结果的可比性。Objective To improve the comparability of HbA1 c results from different detecting systems, to provide the valuable reference for the mutual recognition of HbA1c results among medical laboratories. Methods The fresh mixed whole-blood samples were used for transferring value-assignment and methodology comparison in order to compare the comparability of two methods, namely the Latex enhanced turbid-metric Immunoassay （LETIA） and the High performance liquid chromatography （HPLC）, before and after transferring value-assignment. Results Compared with HPLC method, after transferring value-assignment, the LETIA method made the bias at medical decision level decreased from 8. 83% to 0. 65%. Conclusion The comparability of HbA1 c results is improved by transferring value-assignment and methodology comparison with the fresh mixed whole-blood samples.

关 键 词：糖化血红蛋白 检测系统 方法学 比对 偏倚 

分 类 号：R446.1[医药卫生—诊断学]