补阳还五汤质量标准研究  

Quality standard for Buyang Huanwu Decoction

作　　者：张星[1,2] 陆兔林[1,2] 毛春芹[2] 蔡宝昌[1] 王菊[1] 胡颖菲[1] 王会娟[1] 王蕾[1] 

机构地区：[1]南京中医药大学药学院,江苏南京210046 [2]南京中医药大学江苏省中药炮制重点实验室,江苏南京210046

出　　处：《中成药》2013年第3期529-533,共5页Chinese Traditional Patent Medicine

基　　金：国家中医药管理局中医药行业科研专项(国家发改委)"临床中药汤剂煎煮技术规范化研究"(201007010)

摘　　要：目的建立补阳还五汤(黄芪、当归尾、赤芍、川芎、红花、桃仁、地龙)的质量标准。方法采用薄层色谱(TLC)法对补阳还五汤中的黄芪、当归、赤芍、川芎进行定性鉴别,应用HPLC对芍药苷、毛蕊异黄酮葡萄糖苷、槲皮素进行定量测定。结果薄层色谱斑点清晰,分离度良好,阴性样品无干扰;芍药苷、毛蕊异黄酮葡萄糖苷及槲皮素在测定范围内均表现出良好的线性(r>0.999),方法平均回收率在97.23%～101.65%之间。结论定性定量的方法简便、专属性强、结果准确可靠,所建的标准可用于补阳还五汤的质量控制。AIM To establish the quality standard for Buyang Huanwu Decoction（Astragali Radix,Angelicae sinensis Radix extremitas,Paeoniae Radix rubra,Chuanxiong Rhizoma,Carthami Flos,Persicae Semen,Pheretima）.METHODS TLC was used for the qualitative identification of Astragali Radix,Angelicae sinensis Radix,Paeoniae Radix rubra and Chuanxiong Rhizoma.HPLC was used to determine the contents of paeoniflorin,calycosin glucosidase and quercetin.RESULTS TLC spots were clear and well separated without interference in the negative reference.The compounds showed a good linearity（r＆gt;0.999） in a determination range.The average recovery was between 97.23%-101.65%.CONCLUSION The method is simple,reliable and accurate with good precision.It can be used for the quality control of Buyang Huanwu Decoction.

关 键 词：补阳还五汤 质量标准 HPLC 芍药苷 毛蕊异黄酮葡萄糖苷 槲皮素 薄层色谱法 

分 类 号：R927.2[医药卫生—药学]