全血手工制备浓缩血小板后的血浆再制备冷沉淀的质量评价  

Quality of cryoprecipitate prepared from the plasma after separation of platelets by buffy coat method

作　　者：易中梅[1] 王红苹[1] 李晨晨[1] 王世春[1] 王泽蓉[1] 黎儒青[1] 郭辉[2] 赵树铭[1] 

机构地区：[1]第三军医大学西南医院输血科中国人民解放军重庆血站,重庆400038 [2]第三军医大学西南医院检验科

出　　处：《中国输血杂志》2013年第2期117-120,共4页Chinese Journal of Blood Transfusion

摘　　要：目的评价室温新鲜全血白膜法制备浓缩血小板后的血浆再制备冷沉淀的质量。方法实验组为24例,新鲜全血(400 mL)置室温于<8 h用白膜法制备浓缩血小板后所得的血浆,冰冻保存。对照组1为12例,常规制备新鲜冰冻血浆,冰冻保存。对照组2为12例,新鲜冰冻单采血浆,血浆单采完毕分装为200 mL/袋并立即冰冻保存。3组血浆按常规制备冷沉淀,评价其质量:外观、凝血因子FⅧ及Fib的含量;血细胞残留量。结果 3组冷沉淀外观均正常;WBC含量在3组间无统计学意义。与对照组1比较:实验组凝血因子FⅧ(81.76±34.07)IU较低,Fib(202.63±48.58)mg及Plt(7.81±5.81)×109均较高。与对照组2比较:实验组凝血因子FⅧ含量相当,Fib(202.63±48.58)mg较高、Plt(7.81±5.81)×109较低。结论全血来源的制备浓缩血小板后的冰冻血浆还可以用于冷沉淀的制备,其质量符合国家标准。Objective To investigate the quality of cryoprecipitate prepared from plasma after preparation of buffy coat derived platelet concentrats. Methods The experimental group consisted of 24 cryoprecipitates prepared from plasma after platelet concentrates were prepared by buffy coat method, using whole blood stored at room temperature for less than 8 h. Control group 1 consisted of cryoprecipitates prepared from regular fresh frozen plasma,prepared from whole blood stored at 4 ~C within 8 h of whole blood collection. Control group 2 consisted of cryoprecipitates prepared from apheresis plasma fro- zen within 2 h of collection. All the plasma units were frozen for 3 d before preparation of cryoprecipitate. The quality of cryoprecipitate was evaluated by appearance,F~ and fibrinogen （Fib） content,and residual blood cells. Results The cryoprecipitates of all 3 groups showed normal appearance. There was no difference in residual WBC counts between the three groups. Compared to control group 1 ,the clotting factor FVIII in the experimental group was lower,but the Fib and platelet counts were higher. There was no difference in FV~ between the experimental group and control group 2. Howev er, Fib level was obviously higher and platelet count was significantly lower in the former group. Conclusion The quality of cryoprecipitate prepared from plasma after preparation of platel6t concentrats from whole blood stored at room temperature within 6 h of whole blood collection could meet the national criteria.

关 键 词：白膜法 冷沉淀凝血因子 质量研究 

分 类 号：R457.1[医药卫生—治疗学] R331.143[医药卫生—临床医学]