白膜放置时间对制备手工浓缩血小板质量的影响  

Effect of holding buffy coats before platelet preparation on the quality of platelet concentrates

作　　者：刘凤君[1] 蒋秋容[1] 王泽蓉[1] 王世春[1] 张红[1] 陈敏[1] 贾睿平[1] 赵树铭[1] 

机构地区：[1]第三军医大学西南医院输血科中国人民解放军重庆血站,重庆400038

出　　处：《中国输血杂志》2013年第2期120-122,共3页Chinese Journal of Blood Transfusion

摘　　要：目的评价全血当天分离的白膜(BC)在不同时间分离制备的浓缩血小板(PC)的质量,为手工制备PC提供参考。方法将80袋400 mL全血于采集6 h内分离出BC,将5袋同血型BC由无菌接驳机对接合并成1袋(1个治疗量)后,再均分在3个血小板保存袋内,1袋即刻(0 h组)轻离心分离制备PC,另2袋在22℃血小板保存箱分别振摇4 h(4 h组)和16 h(16 h组)后再分离PC。对所有标本留样进行血小板质量检测,包括Plt、血小板回收率、CD62P、聚集率、RBC混入量、WBC混入量、FHb含量。结果 3组PC制剂RBC混入量、Plt、血小板回收率、CD62P、聚集率差异无统计学意义;WBC混入量:0 h(6.76±1.29)和4 h组变化不明显,16 h(3.78±0.45)组降低明显(P<0.05);FHb含量:随BC处理时间延长有增高趋势,16 h(65.62±11.11)与0 h(33.45±6.95)比差异具统计学意义(P<0.05)。结论随BC放置时间延长对制备PC制剂的质量有一定影响。Objective To evaluate the quality of platelet concentrates （PCs） prepared from buffy coats held for different time periods. Methods Buffy coats （BCs） （ n = 80） were prepared from 400 mL whole blood （WB） units within 6 h of collection. Every 5 BCs were pooled and then each pool were divided into three equal bags. One bag was taken to prepare PCs immediately （0 h group） ,and the other two bags of BCs were agitated in platelet incubator,and agitated for 4 h （4 h group） ,and 16 h （16 h group） at 22~C before PC preparation. The in vitro quality of platelets was investigated. Results Platelet,RBC counts showed little difference between these groups. In comparison to 0 h and 4 h groups, the decrease d WBC counts in the 16 h group was significant. Free hemoglobin increased as the agitation time extended. Conclusion The agitation time of BCs may affect the quality of platelet concentrates.

关 键 词：白膜法 浓缩血小板 制备 血小板 质量 

分 类 号：R457.1[医药卫生—治疗学] R331.143[医药卫生—临床医学]