低温对椰心叶甲啮小蜂保护酶活性的影响  

Effect of Low Temperature on the Protective Enzymes in Tetrastichus brontispae

作　　者：郭东峰[1,2] 吕宝乾[2] 韦曼丽[2] 金启安[2] 温海波[2] 彭正强[2] 

机构地区：[1]海南大学环境与植物保护学院,海南海口570228 [2]中国热带农业科学院环境与植物保护研究所农业部热带农林有害生物入侵监测与控制重点开放实验室海南省热带农业有害生物监测与控制重点实验室,海南儋州571737

出　　处：《热带作物学报》2013年第2期331-334,共4页Chinese Journal of Tropical Crops

基　　金：留学人员科技活动择优资助项目(2011年度);国家自然科学基金(31101496);973计划子课题(No.2009CB119206);公益性行业计划子课题(No.200903026-5);国家科技合作专项(2011DFB30040)

摘　　要：采用分光光度计法测定低温处理后椰心叶甲啮小蜂(Tetrastichus brontispae)成虫体内3种保护酶活性的变化。结果表明,椰心叶甲啮小蜂成蜂受到低温胁迫后体内CAT、POD和SOD分别在10℃和-2℃出现两个高峰,酶活性总体水平高于对照。-2℃时,3个处理时间的CAT、POD和SOD活性最高,其中,CAT、SOD活性差异性显著。由于各种酶的特性不同,它们对低温应激处理时间的反应亦不同,SOD在处理12 h时酶活性最高,而CAT与POD在24 h时酶活性最高。The activity of protective enzymes in adult of Tetrastichus brontispae were detected with the method of spectrophotometric. The conclusion showed that the activity of all the three enzymes of CAT, POD and SOD had two peaks in the range of 10℃ and -2 ℃ when the adults of Tetrastichus brontispae suffered low temperature stress. Additionally, the whole activity of the three enzymes in the condition of dealing circumstances was higher than the 25℃. When in the temperature of -2℃, CAT, POD and SOD activity were all the highest Of the three processing, and there were significant differences on CAT and SOD activity （p 〈0.05）. For their own various characteristic of each enzyme, they shared different optimum dealing time in the field of low temperature stress, 12 h for SOD compared with 24 h for CAT and POD.

关 键 词：低温胁迫 椰心叶甲啮小蜂 过氧化氢酶 过氧化物酶 超氧化物歧化酶 

分 类 号：S433[农业科学—农业昆虫与害虫防治]