腺嘌呤对电离辐射诱发AHH-1细胞凋亡的保护作用(英文)  

Radioprotective effect of adenine on irradiation-induced apoptosis

作　　者：王小彦[1,2] 马增春[1] 邵帅[1] 洪倩[1] 王宇光[1] 谭洪玲[1] 路晓钦[2] 董志[2] 高月[1] 

机构地区：[1]军事医学科学院放射与辐射医学研究所,北京100850 [2]重庆医科大学药学院,重庆400016

出　　处：《中国天然药物》2013年第2期139-144,共6页

基　　金：supported by the National Natural Sciences Foundation of China (Nos. 81073028, 81130067, 81274127);National Science and Technology Support Task (No. 2008BA151B02)~~

摘　　要：目的:观察腺嘌呤对淋巴母细胞AHH-1辐射损伤的保护作用并探讨其作用机制。方法:照射前12h给予不同浓度的腺嘌呤,再经4.0Gy60Coγ射线照射诱发细胞凋亡,采用MTS法计算细胞存活率;AnnexinV-FITC/PI双染法、Caspase3/7发光法检测细胞凋亡率;DNALadder法观察凋亡断裂条带;RT-PCR分析细胞Caspase-9、Bax、Bcl-2mRNA表达的变化。结果:与正常组比较,电离辐射后细胞存活率79.4%显著下降(P<0.01,加入0.001,0.01,0.1μmol·L–1腺嘌呤细胞存活率分别为85.4%,89.6%,92.2%明显升高(P<0.01),且随作用浓度的增加而增大,DNA断裂片段也减少,细胞中Caspase-9、Bax表达明显减少,Bcl-2表达明显增加,凋亡的各项指标均明显改善。结论:腺嘌呤对电离辐射诱发的细胞凋亡有保护作用,并且对细胞凋亡的抑制呈剂量依赖正相关关系。AIM： To investigate the radioprotective effect of adenine on irradiated lymphocytes and discover the possible mechanisms of protection. METHODS： Lymphocytes were pretreated for 12 h with adenine （0.001-0.1 pmol.L-1） and then exposed to 4 Gy radiation. Cell viability was observed by the MTS assay, apoptosis was detected by Annexin V-FITC/PI, DNA ladder, and caspase 3/7 activity. Caspase-9, Bax, and Bcl-2 gene expression was investigated by RT-PCR. RESULTS： Irradiation increased cell death and DNA fragmentation. Pretreatment with adenine significantly reversed this tendency. Furthermore, several anti-apoptotic characteristics of adenine were determined, including the ability to inhibit caspase 3/7, upregulate B-cell lymphoma （Bcl-2） and downregulate Bcl-2- associated X （Bax）, capase-9 gene expression in 4 Gy-irradiated AHH-1 cells. CONCLUSION： The results suggest that adenine had a radioprotective effect to inhibit apoptosis in a concentration dependent manner.

关 键 词：腺嘌呤 淋巴母细胞AHH-1 细胞凋亡 电离辐射 

分 类 号：R965[医药卫生—药理学]