毛茛总苷对人胃癌细胞和胰腺癌细胞增殖作用的初步研究  

Anti-proliferation Effect of Total Glycosides of Ranunculus Japonicus on Gastric Cancer Cells and Pancreatic Cancer Cells of Human in Vitro

作　　者：朱峰妍[1,2] 傅士龙[2] 成旭东[1] 曹志飞[2] 蒋小岗[1] 鲍美美[2] 周泉生[2] 顾振纶[1] 

机构地区：[1]苏州大学,苏州中药研究所,江苏苏州215007 [2]苏州大学,唐仲英血液学研究中心,江苏苏州215123

出　　处：《抗感染药学》2013年第1期24-28,共5页Anti-infection Pharmacy

基　　金：苏州市科技支撑计划项目(编号:SS201004)

摘　　要：目的:研究毛茛总苷体外对人胃癌细胞MGC803和人胰腺癌PATU898细胞增殖、细胞周期的影响。方法:采用Alamar Blue和瑞-姬染色方法检测毛茛总苷对人胃癌细胞MGC803和人胰腺癌细胞PATU8988增殖的作用;PI染色法观察毛茛总苷对MGC803和PATU8988细胞周期的影响;Annexin V/PI双染实验法检测毛茛总苷对MGC803细胞凋亡的影响。结果:用Alamar Blue检测及瑞-姬染色法,其结果显示毛茛总苷对人胃癌细胞MGC803和人胰腺癌细胞PATU8988表现出具有较好地增殖和抑制作用,其48h的半数抑制浓度分别为222.05μg/mL,341.23μg/mL;用PI单染法,其结果显示毛茛总苷对2株肿瘤细胞的细胞周期无明显影响;用Annexin V/PI双染法,其实验结果显示毛茛总苷可诱导MGC803细胞凋亡。结论:毛茛总苷在体外可以明显抑制MGC803和PATU8988的增殖,其作用机制可能与诱导细胞凋亡有关,而与细胞周期阻滞无关。Objective： To observe and to detect the effect of total glycosides of Ranunculusjaponicus on human gastric cancer cell and pancreatic cancer cell＇s proliferation and cell cycle in vitro. Methods： The effects of total glycosides of Ranunculusjaponicus on human gastric cancer cell and pancreatic cancer cell＇s proliferation were observed by Alamar Blue assay and Wright-Giemsa assay. PI assay was used to detect the effect of total glycosides of Ranunculusjaponicus on the cell cycle distribution of MGC803 and PATU8988. Annexin V/PI double staining assay was used to detect the cell apoptosis of MGC803. Results： AlamarBlue and Wright-Giemsa staining results showed that that total glycosides of Ranunculus japonicus could obviously inhibit MGC803 and PATU8988 proliferation and the IC50 at 48h was about 222.05 μg/mL and 341.23 μg/mL. The flow cytometry PI staining assay results showed that total glycosides of Ranuncu- lusjaponictts have little effect on the MGC803 and PATU8988 cell cycle arrest. The Annexin V/PI double staining assay results tell us that the total glycosides of Ranunculusjaponicus can induce MGC803 apoptosis. Conclusion： Total glycosides of Ranunculusjaponicus could effectively inhibite human gastric cancer cell MGC803 and pancreatic cancer cell PATU8988 proliferation. The mechanism of it is associated with cell apoptosis, but not with cell cycle arrest.

关 键 词：毛茛总苷 胃癌 胰腺癌 MGC803 PATU8988 细胞凋亡 

分 类 号：R965[医药卫生—药理学]