肠道传播的非甲非乙型(戊型)肝炎病毒基因克隆的初步报告  

A PRELIMINARY REPORT ON THE GENETIC CLONING OF HEPATITIS E VIRUS (HEV)

作　　者：毕胜利[1] 刘崇柏[1] 汪媛[1] 赵洪兰[1] 曹学义[2] 王光明[3] 庄辉[4] 

机构地区：[1]中国预防医学科学院病毒学研究所 [2]新疆维吾尔自治区卫生防疫站 [3]中国医学科学院实验动物研究所 [4]北京医科大学

出　　处：《病毒学报》1991年第1期66-68,共3页Chinese Journal of Virology

摘　　要：肠道传播的非甲非乙型肝炎是通过粪口途径传播的。在南亚、中亚细亚和东南亚诸国,东非及我国新疆南部地区都曾发生过大流行,对人民健康危害极大。 该病的病毒(HEV)为直径35nm的廿面体,核酸为正链单链RNA。病毒对理化因子的抵抗力明显不同于肠道病毒,在粪便中极易降解,在常温和-20℃也很快裂解,在组织培养上尚不能繁殖。在一般粪便标本中,HEV的量和RNA含量都较低。因此,用常规的聚合酶链反应(PCR)不能从粪便标本中获得HEV的基因放大物。最近,美国基因实验室(Genelab.)建立了核酸序列非依赖性基因放大法(Sequence independent singleUsing the sequence independent single primer gene amplification method a 2,000bp 3' cDNA clone was isolated from the faeces collected from a acutely infected Chinese HEV patient and from the faeces, liver tissue of experimentally infected Rhesus monkey.The specificity of the clone isolated was confirmed by probes Hp1, Hp2, pF2 and pR3, which represented the more conservative sequences in p part of the HEV genome cloned from the faeces of a Burmese HEV patient.It was demonstrated that the SISpA products could be hybridized with all four probes,except that the pR3 probe gave a weaker reaction with the SISpA products.

关 键 词：非甲非乙肝炎 基因克隆 HEV 

分 类 号：R512.630.3[医药卫生—内科学]