人乳头瘤病毒16型E6-E7基因体外转化NIH3T3细胞  

TRANSFORMATION OF NIH3T3 CELLS IN VITRO WITH HUMAN PAPILLOMA VIRUS TYPE16 E6-E7 GENES

作　　者：金顺钱[1] 孙亚洲[1] 张伟[1] 梁克[1] 明利华[1] 王晓宏 王希霞[1] 梁肖[1] 侯芷瑛 商铭 

机构地区：[1]中国医学科学院肿瘤研究所

出　　处：《病毒学报》1991年第2期97-101,共5页Chinese Journal of Virology

摘　　要：把人乳头瘤病毒16型(HPV16)长控制区(LCR)及与其相连的E6—E7基因片段正向插入pSV2neo质粒,构建HPVl6 LCR E6-E7真核细胞表达质粒,转染NIH3T3细胞,用 G418作筛选药物。转化的细胞形态具有表型改变,出现非锚地依赖性生长及在裸鼠体内致肿瘤能力。Southern印迹杂交结果表明,重组质粒已整合于转化细胞的染色体上。A fragment containing HPV16 E6-E7 ORFs with its autologous Long Control Region (HPV16 LCR E6-E7) was inserted into eukaryotic expression vector pSV2neo in sense orientation. We successfully transformed NIH3T3 cells in vitro with the constructed plasmid. The transformed cells showed anchorage-independent growth and can cause tumor in nude mice. Southern blot analysis of DNAs from the transformed cells and tumor tissues in nude mice showed integration of the constructed plasmid into the host DNA. The results indicated that HPY16 E6-E7 transforming genes might play a critical role in the development of human cervical carcinoma.

关 键 词：人乳头瘤病毒 NIH3T3细胞 转化 

分 类 号：R373.9[医药卫生—病原生物学]