用ELISA法快速诊断柯萨奇B组病毒感染的儿童心肌炎  

APPLICATION OF INDIRECT-ELISA AND CAPTURE ELISA FOR RAPID DIAGNOSIS OF COXSAKIE B VIRUS INFECTION IN CHILDREN WITH MYOCARDITIS

作　　者：江永珍[1] 张礼璧[1] 谢景石 朱晖[1] 李继芬 王棣华[2] 

机构地区：[1]中国预防医学科学院病毒学研究所 [2]河南医科大学附属第三医院 [3]首都儿童医院临床实验室

出　　处：《病毒学报》1991年第2期158-163,共6页Chinese Journal of Virology

摘　　要：以柯萨奇B组(Cox B)1～5型病毒感染的Vero细胞上清液作为抗原,建立了间接ELISA法和IgM抗体捕捉ELISA法(MacELISA),并分别用于检测心肌炎和非心肌炎患儿血清中Cox B1～5型病毒IgG和IgM抗体。结果IgG类抗体滴度≥6 400者,在80例心肌炎(包括30例急性心肌炎)患儿中有49例,占61.25％,50例非心肌炎患儿中有11例,占22％。两者有显著性差别,u=5.6,P<0.01。而IgM抗体的阳性率,急性心肌炎患儿为73％,心肌炎患儿为32％,总阳性率为47.5％;而非心肌炎患儿仅为8％。30例心肌炎和急性心肌炎IgM抗体阳性者中,以抗Cox B3型的百分率最高,占40％(12例);其次为2型,占33％(10例);4型占17％(5例),1型占6.6％(2例):5型占3.3％(l例)。10例急性心肌炎患儿的双份血清,间接ELISA法检测恢复期IgG抗体呈4倍以上升高或降低者有6例(60％),MacELISA法检测IgM抗体8例(80％)阳性。The conventional virus isolation and neutralization test for diagnosis Cox B virus infection were difficult for practical use. We used crude viral antigen prepared from Cox B1-5 virus infected Vero cell and developed on indirect-ELISA and μ-antibody capture ELISA test for the detection of Cox Bl-5 virus antibodies. 10 cases with paired sera and 80 cases with single sera from myocarditis/acute myocarditis and 50 control sera from res-poratory infection and aseptic meningitis were test. All sera were collected from children in the 1-15 years age group, between May and December 1989.Of the 10 cases with paired sera, 6(60% ) had IgG 4-fold rise or fall by indirect-ELISA and 8(80%) were IgM positive by MacELISA. 80 cases of myocarditis sera and 50 cases control sera were examined. The percentages of Cox B IgG titres≥6,400 were 61.25% and 22% respectively, and the IgM positive rates were 47.5% and 8% respectively. The type distribution of IgM positives with myocarditis/acute myocarditis were 7% against Cox B1, 33% against Cox B2, 40% against Cox B3, 17% against Cox B4, 3% against Cox B5.

关 键 词：柯萨奇B病毒 ELISA 儿童 心肌炎 

分 类 号：R373.9[医药卫生—病原生物学]