白英生物碱通过激活FAS途径诱导人肺癌A549细胞凋亡的研究  

Solanum lyratum Thunberg Alkaloid Induces Human Lung Adenoearcinoma A549 Cells Apoptosis by Activating FAS-pathway

作　　者：涂硕[1] 万福生[1] 韦星[2] 农嵩[2] 朱伟锋[1] 刘卓琦[1] 

机构地区：[1]南昌大学医学院,江西南昌330006 [2]右江民族医学院,广西百色533000

出　　处：《时珍国医国药》2013年第1期66-68,共3页Lishizhen Medicine and Materia Medica Research

基　　金：江西省科技攻关计划项目(No.060004);广西青年科学基金项目(No.桂科青0640074);广西教育厅科研项目(No.200710MS053)

摘　　要：目的探讨白英生物碱(STA)诱导人肺癌A549细胞凋亡及其对Fas途径相关基因表达的影响。方法采用STA处理体外培养的A549细胞,用MTT法检测细胞增殖抑制率,Annexin V-FITC/PI双染后用流式细胞仪检测细胞凋亡,Western blot检测Fas、FasL、Cleaved caspase-8和Cleaved caspase-3蛋白表达。结果 STA能抑制A549细胞增殖,STA各组细胞抑制率显著升高且呈剂量依赖性,与正常对照组比较差异有统计学意义(P<0.01);STA各组细胞凋亡率明显升高,与正常对照组比较差异有统计学意义(P<0.01);Caspase-8/Caspase-3抑制剂能抑制STA诱导细胞凋亡,与同剂量STA组比较差异有统计学意义(P<0.05或P<0.01);STA各组细胞Fas、FasL、Cleaved caspase-8和Cleaved caspase-3表达显著上升,与正常对照组比较差异有统计学意义(P<0.05或P<0.01)。结论 Fas介导的死亡受体途径参与STA诱导肺癌A549细胞凋亡。Objective To investigate the effects of solanum lyratum thunberg alkaloid（STA） on induction of apoptosis and the ex- pression of Fas - pathway related genes in A549 cells. Methods A549 cells were treated with STA in vitro. The proliferation inhib- itory rate was evaluated by MTI＂ assay. Induction of cell apoptosis rate was determined by flow cytometry（FCM） method after An- nexin V - FITC/PI double staining. The expression of Fas, FasL, Cleaved caspase - 8 and Cleaved caspase - 3 were detected by western blot. Results STA could inhibit the proliferation of A549 cells. The inhibitory rate was increased obviously with dose de- pendency in STA groups, compared with the control group, the difference was statistically significant（ P 〈 0.01 ）. The apoptotic rate was increased significantly in STA groups, compared with the control group, the difference was statistically significant （P 〈 0.01 ）. Caspase - 8/Caspase - 3 inhibitor could suppress the apoptosis of A549 cells induced by STA, compared with the same dose of STA group,the difference was statistically significant（ P 〈 0.05 or P 〈 0.01 ）. The expression of Fas, FasL, Cleaved caspase - 8 and Cleaved caspase -3 proteins were increased markedly in STA groups, compared with the control group, the difference was sta- tistically significant（ P 〈 0.05 or P 〈 0.01 ）. Conclusion Fas - mediated death receptor pathway is involved in STA inducing lung adenoearcinoma A549 cells apoptosis.

关 键 词：肺腺癌 白英生物碱 细胞凋亡 死亡受体途径 

分 类 号：R962[医药卫生—药理学]