苦参粗提液中苦参碱和氧化苦参碱的高效液相色谱法测定  

Determination of Matrine and Oxymatrine in the Crude Extract of Sophora flavescens by HPLC

作　　者：于海红[1] 姜音[1] 丛景香[1] 吴秀红[1] 

机构地区：[1]辽宁科技大学化工学院,辽宁鞍山114051

出　　处：《时珍国医国药》2013年第1期126-127,共2页Lishizhen Medicine and Materia Medica Research

基　　金：教育部博士点学科专项科研基金(No.20070146001)

摘　　要：目的建立HPLC法测定苦参粗提液中苦参碱和氧化苦参碱含量的方法。方法使用AichromBond-AQC18色谱柱,以甲醇-0.05%三乙胺水溶液(70∶30,V/V)为流动相,流速为1.00 ml/min,检测波长为215 nm。结果苦参碱和氧化苦参碱分别在0.02～160 mg/L和0.04～100 mg/L范围内,呈良好的线性关系。苦参碱和氧化苦参碱平均加标回收率分别为98.59%和99.43%。结论该方法简单、灵敏、准确、重现性好、分离度高。Objective To develop a method for determinition matrine and oxymatrine of crude extract of Sophoraflavescens. Meth- ods AichromBond -AQ C lS column （4.6 mm x 250 mm,5 ~m）was used as the solid phase. Methanol -0.05 % triethylamine so- lution （70：30, V/V）was taken as mobile phase. The flow rate was 1.00 ml/min. The UV detection wavelength was 215 nm. Re- suits The calibration curves of matrine and oxymatrine were linear in the range of 0.02 - 160 mg/L and 0.04 - 100 mg/L,respec- tively. The average recoveries of both were 98.59% and 99.43% , respectively. Conclusion The method established method is sensitive, simple, and accurate.

关 键 词：苦参 苦参碱 氧化苦参碱 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]