辣椒粉中苏丹红Ⅰ的免疫亲和柱-高效液相色谱法检测  

Detecting Sudan Ⅰ in chill powder by the method of Immunoaffinity Column-HPLC

作　　者：裘雪梅[1] 朱立鑫[1] 徐玲[1] 徐富勇[1] 刘仁荣[1] 

机构地区：[1]江西科技师范学院生命科学学院,江西南昌330013

出　　处：《时珍国医国药》2013年第1期234-235,共2页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金项目(No.30860240);江西省科技厅科技项目(No.2010GZN0022)

摘　　要：目的建立用免疫亲和柱分离净化、高效液相色谱法测定食品中苏丹红I的方法。方法将制备好的苏丹红Ⅰ单克隆抗体与经修饰的琼脂糖凝胶Sepharose-4FF偶联,制成苏丹红Ⅰ免疫亲和柱,样品经乙腈提取,并用PBS缓冲液稀释成20%乙腈-PBS溶液后,过实验室自制的苏丹红Ⅰ免疫亲和柱净化,建立IAC-HPLC法检测苏丹红Ⅰ含量。结果测得辣椒粉中SudanI的回收率为29.12%～90.32%,并随机抽检了市售的及自种的四种辣椒粉,检测结果均为阴性。结论成功制备了苏丹红Ⅰ免疫亲和柱,并建立了免疫亲和柱-HPLC法测定辣椒粉中的苏丹红I的方法。Objective Establishing the method of Immunoaffinity Column - HPLC to detect the Sudan I in food. Methods Cou- pling the Sudan I monoelonal antibody to the agarose gel Sepharose-4FF, then prepare the Sudan I immunoaffinity column, the samples were extracted with acetonitrile, and diluted with PBS buffer to 20% acetonitrile - PBS, then purification with Sudan I immunoaffinity column, the method of IAC - HPLC detection of Sudan I content has been established. Results The chili powder Sudanl recovery was 29.12% to 90.32% , and a random sampling of commercially available, and four chili powder samples test results were negative. Conclusion Successfully prepared Suand I IAC column, and established the method of detecting sudan I in chill powder by IAC - HPLC.

关 键 词：免疫亲和柱 高效液相色谱 苏丹红Ⅰ 

分 类 号：R284.1[医药卫生—中药学]