电针对老年痴呆症大鼠海马CaMKⅡ表达的影响  

Observation on the Effect of Electroacupuneture to the Expression of CaMKⅡin Rat Hippocampus Tissues in Senil Dementia Rats

作　　者：徐秋玲[1] 刘涛[1] 

机构地区：[1]海南医学院,海南海口571101

出　　处：《时珍国医国药》2013年第1期236-238,共3页Lishizhen Medicine and Materia Medica Research

基　　金：海南省自然科学基金(No.310054);海南省卫生厅课题(琼卫2010-45);海南医学院人才引进启动基金(No.2010008)

摘　　要：目的观察电针(EA)老年痴呆症大鼠海马CaMKⅡ表达的的关系。方法 SD雌鼠24只,随机分为假手术组(n=8),模型组(n=8),电针组(n=8),模型组及电针组用切除卵巢颈背部皮下注射D-半乳糖的方法造成记忆损伤的动物模型。电针组电针"百会"-"四神聪"穴,1次/d,连续10 d。采用Morris水迷宫法测试动物记忆行为变化。用免疫组化法测定海马CAMKⅡ阳性产物的表达。结果与模型组比较,电针组大鼠逃避潜伏期显著缩小(P〈0.05);与模型组比较,电针组海马CA1区CaMKⅡ表达明显上调。结论电针"百会"-"四神聪"穴后可使海马CA1区CaMKⅡ表达明显上调,改善了大鼠学习记忆的能力。Objective To observe the effect of electroacupunoture （EA） to the expression of Calcium/calmodulin - dependent protein kinase Ⅱ （ CAMK Ⅱ ） in rat hippocampus tissues in senil dementia rats. Methods A total of 24 SD rats were randomized into control, Sham, OVX and OVX ＋ EA groups ,with 8 cases in each. For rats of the OVX and OVX ＋ EA groups, subcutaneous injection of D -galactose was given once daily for 7 W. EA（2/15Hz, 1 -2 mA） was applied to ＂baihui＂ - ＂sishengcong＂, for 30min, once daily Morris water maze test was conducted to evaluate the rats＇memory - learning ability. CAMK Ⅱ expression of hip- pocampus was detected by immunohistochemistry. Results Compared with Sham group, escape latency shorten significantly after EA （P 〈 0.05 ） ; CAMK Ⅱ immunoreaction positive product integral grey values （IGV） in rat hippocampus of OVX ＋ EA groups were significantly lower （ upregulation of expression） than OVX group （ P 〈 0.05 ）. Conclusion EA ＂baihui＂ - ＂sishengcong＂ has an effect To improve the ability of learning and memory in rats which is closely related to the upregulation of CAMK Ⅱ expression.

关 键 词：电针 老年痴呆 钙调素依赖性蛋白激酶Ⅱ 

分 类 号：R2-03[医药卫生—中医学]