Chiralpak IB手性固定相拆分消旋卡多曲光学异构体  

Chiral Separation of Racecadotril on Chiralpak IB Chiral Stationary Phase

作　　者：白瑞霞[1] 

机构地区：[1]郑州市第一人民医院,河南郑州450004

出　　处：《中国医药工业杂志》2013年第3期286-289,共4页Chinese Journal of Pharmaceuticals

摘　　要：建立了一种用纤维素手性固定相拆分消旋卡多曲光学异构体的高效液相色谱方法。采用纤维素-三(3,5-二甲基苯胺基甲酸酯)手性固定相(Chiralpak IB),考察醇类添加剂种类及比例、柱温和流速对拆分的影响。流动相为正己烷-异丙醇(60︰40),流速0.6 ml/min,柱温25℃,检测波长210 nm,卡多曲两个异构体在Chiralpak IB手性柱上能够达到基线分离,在0.01～0.5 mg/ml线性关系良好,平均回收率为99.7%和99.6%,RSD为0.79%和0.91%。An HPLC method with cellulose tris （3,5-dimethylphenylcarbamate） as chiral stationary phase （Chiralpak IB） was established for the chiral separation of racecadotril enantiomers. The influences of the mobile phase composition, column temperature and flow rate on the resolution were investigated. A complete resolution was achieved under the following conditions： hexane-isopropanol （60 ： 40） as the mobile phase at the flow rate of 0.6 ml/min, column temperature of 25 ℃ and detection wavelength of 210 nm. The calibration curves of cadotril enantiomers were linear in the concentration range of 0.01 - 0.5 mg/ml. The average recoveries were 99.7% and 99.6%, with RSDs of 0.79% and 0.91%, respectively.

关 键 词：卡多曲 高效液相色谱 纤维素 手性分离 

分 类 号：R917[医药卫生—药物分析学]