大蓟-魁蓟-覆瓦蓟的蒙花苷和大蓟苷含量比较  

Content comparison of buddleoside and pectolinarin in Cirsium japonicum,C.leo and C.leducei

作　　者：李治昊[1,2] 宋良科[1] 王小宁[1] 王岩[1] 吴蜀星[1] 余成龙[1] 宗玉英[3] 

机构地区：[1]西南交通大学生命科学与工程学院,四川成都610031 [2]成都中医药大学峨眉学院,四川峨眉614201 [3]香港中文大学中医学院

出　　处：《中国中药杂志》2013年第5期674-677,共4页China Journal of Chinese Materia Medica

基　　金：科技部自然科技基础条件平台建设子项目(2005DKA21404);国家中医药管理局行业科研专项(201107009)

摘　　要：目的:通过对大蓟与主要类似品魁蓟、覆瓦蓟的性状与蒙花苷、大蓟苷含量比较,为研究药材大蓟的质量控制方法奠定基础。方法:实地采样并购买对口药材,将样本分为根、茎、叶、花与花序4部分,用HPLC测定蒙花苷、大蓟苷含量;色谱柱Waters XBridgeTMC18(4.6 mm×250 mm,5μm);流动相甲醇-水(45∶55);检测波长326 nm;流速0.8 mL.min-1;柱温30℃。结果:蒙花苷的标准曲线方程为Y=74 064X-47 748,R2=0.991,大蓟苷的标准曲线方程为Y=171 164X-180 707,R2=0.999,线性关系良好。大蓟叶蒙花苷质量分数为1.987 3%、魁蓟叶为1.412 2%、覆瓦蓟叶为0.149 2%,大蓟与魁蓟的根、茎中蒙花苷质量分数均较低,覆瓦蓟根未检出蒙花苷。大蓟苷在大蓟与魁蓟各部位均未检出,覆瓦蓟各部位中以叶的质量分数最高为0.069 0%。结论:大蓟,魁蓟,覆瓦蓟均为菊科蓟属植物,药材性状十分相似,覆瓦蓟在四川做大蓟用可能与叶缘、叶面有针刺并能检测出大蓟苷(柳穿鱼叶苷)有关。魁蓟在陕西,四川、河南部分地区做大蓟用,可能与叶色、被毛、叶缘针刺以及粉末特征与大蓟药材记述相似有关。Objective： To compare Cirsium japonicum characteristics with C. leo and C. leducei,along with the content of bud- dleoside and pectolinarin, and lay the foundation for the quality control of C. japonicum. Method ： Samples were collected and the rele- vant drugs were bought. The samples were divided into root, stem,leaf and flower, and the content of buddleoside and pectolinarin was determine by the HPLC. Chromatographic column： Waters XBridge TM C18 （4. 6 mm × 250 mm） , mobile phase： methanol-water （45： 55） , measurement wavelength： 326 nm, flow rate： 0. 8 mL · min-1, column temperature： 30 ~C. Result and Condusion： Standard curve equation of buddleoside ： Y = 74 064X-47 748,R2 = 0. 991. Standard curve equation of peetolinarin： Y = 1 711 64X - 180 707, R2 =0. 999. The content of buddleoside： C. japonicum leaf was 1. 987 3% , C. leo leaf 1. 412 2% , C. leducei leafO. 149 2%. The content of buddleoside was lower in root and stem. Pectolinarin was not detected in the C. japonicum and C. leo. The pectolinarin con- tent was 0. 069 0% in C. leducei leaf.

关 键 词：大蓟 类似品 性状鉴定 蒙花苷 大蓟苷 

分 类 号：R284[医药卫生—中药学]