用重组Epstein-Barr病毒早期蛋白P83为抗原检测鼻咽癌病人血清中IgA抗体  

DETECTION OF IgA ANTIBODY IN SERA FROM PATIENTS WITH NASOPHARYNGEAL CARCINOMA USING RECOMBINANT EARLY PROTEIN P83 OF Epstein-Barr ( EB ) VIRUS

作　　者：纪志武[1] 方仲[1] 曾毅[1] 

机构地区：[1]中国预防医学科学院病毒学研究所

出　　处：《病毒学报》1991年第3期269-271,共3页Chinese Journal of Virology

摘　　要：以重组EB病毒早期蛋白P83为抗原,用Western blot检测了135份鼻咽癌病人和100份正常人血清中IgA/P83抗体,阳性率分别为96％和0,以常规的间接免疫酶法检测这两种血清中IgA/EA抗体,阳性率分别为71％和0。IgA/P83抗体的几何平均滴度较IgA/EA高4倍以上。这表明,用Western blot查IgA/P83敏感、特异,可替代用间接免疫酶法查IgA/EA用于鼻咽癌的早期诊断。The IgA antibody to EB virus early antigen in 135 sera of patients with NPC and 100 sera of normal individuals was detected by Western blot assay using 83kD recombinant early protein ( P83). The positive rate of IgA/P83 was 96% and 0% respectively. The positive rate of IgA /EA in these two groups was 71% and 0% respective by immunoenzyma-tic test. The antibody geometric mean titer(GMT) of IgA/P83 was four times higher than that of IgA/EA.The conclusion is that for diagnosis of NPC, detection of IgA/P83 in sera by Western blot is better than IgA/EA by indirect immunoenzy-matic test.

关 键 词：EB病毒 IGA抗体 鼻咽癌 早期蛋白 

分 类 号：R373.9[医药卫生—病原生物学]