流行性出血热病毒R22株M片段克隆及序列分析  

MOLECULAR CLONING AND SEQUENCING OF THE M GENOME SEGMENT OF EPIDEMIC HEMORRHAGIC FEVER VIRUS R22 STRAIN

作　　者：石立成 杭长寿[1] 李德新[1] 袁劲松[1] 金冬雁[1] 宋干[1] 

机构地区：[1]中国预防医学科学院病毒学研究所

出　　处：《病毒学报》1991年第4期295-302,共8页Chinese Journal of Virology

基　　金：国家863高技术计划生物技术领域资助项目

摘　　要：用标准的cDNA合成与多聚酶链式反应(PCR)相结合的方法,克隆了我国家鼠型出血热病毒R22株M片段cDNA,完成了全部核苷酸序列分析。病毒M片段由3656个核苷酸组成,病毒互补的RNA由一个单一的开放读码框架组成,编码1134个氨基酸。其核苷酸序列与SR-11、Hantaan、HallnasB1病毒相比同源性分别为95％、76％和52％,推导的氨基酸序列同源性为96％、74％和53％。在R22G1编码的序列中有5个糖基化位点,全部与SR-11保守,与Hanta-an和HallnasB1分别有4个和3个保守,G2编码的序列中有1个糖基化位点,4株病毒全部保守,而且4株病毒M片段在其开放读码框架内的半胱氨酸残基高度保守,亲、疏水基团的分布也表现出很高的相似性,提示G1和G2蛋白可能具有相似的结构与功能。The M genome RNA segment of epidemic hemorrhagic fever virus R22 strain was characterized by molecular cloning with reverse transcriptase and polymerase chain reaction ( RT-PCR ) techniques, and the nucleotide sequences of the corresponding cDNA clones were determined. The virion M segment RNA is 3653 bases long with 3'-and 5'-terminal complementary 20 bases. A single long open reading frame ( ORF ) in the viral complementary-sense RNA had the potential to encode 1134 amino acids or a polypep-tide of 126,000D. Comparisons of the R22 M segment to that of SR-11, Hantaan and Hallna's B1 reveal 95%, 76% and 52% sequence homologies at the nucleotide level and 96%, 74% and 53% at the deduced amino acids level respectively. Five potential asparagine-linked glycosylation sites of the encoded gly-coprotein were contained within the G1 amino acid sequence, all were conserved with SR-11,but four and three of which were conserved with Hantaan and Hallnas B1 viruses. One glycosylation site was contained within the G2 amino acid sequence, all were conserved with the four viruses. Furthermore, all cysteine residues were highly conserved within the M segment ORFs of all four viruses and of hydrophilicity profiles of the four segments are stri-ckingly similar indicating that they are very similar in structures and functions.

关 键 词：出血热 流行性 病毒 克隆 序列分析 

分 类 号：R373.32[医药卫生—病原生物学]