痘苗病毒载体介导的反义RNA抑制HBsAg基因表达的研究  

VACCINIA VIRUS VECTOR-MEDIATED ANTISENSE RNA INHIBITION OF HBsAg EXPRESSION IN CELL CULTURE SYSTEM

作　　者：赵小侠[1] 曹雷[1] 毛冬丽 张爱臣 王平[1] 侯云德[1] 

机构地区：[1]中国预防医学科学院病毒学研究所病毒基因工程国家重点实验室

出　　处：《病毒学报》1991年第4期309-314,共6页Chinese Journal of Virology

基　　金：国家863高技术计划生物技术领域资助项目~~

摘　　要：将含有乙型肝炎病毒表面抗原(HBsAg)基因的DNA片段,按转录的相反方向插入痘苗病毒P11启动子下游,构建成重组质粒pJ16/HBS和pHBS-1。使用磷酸钙沉淀技术将这些质粒转染能分泌HBsAg并已被痘苗病毒感染的细胞,观察到HBsAg的合成被特异性地抑制,抑制率最高达95％以上。Recombinant plasmids were constructed by the insertion of DNA fragments coding HBsAg gene in opposite orientation with respect to the vaccinia virus promoter Pll. When cells secreting HBsAg previously infected with vaccinia virus, were transfected with these plasmids using calcium phosphate precipitation technique, the synthesis of HBsAg was significantly decreased. The maximal inhibitory rate appeared at 6-12 hrs and was more than 90%.

关 键 词：痘苗病毒 反义RNA HBSAG 基因 

分 类 号：R373.9[医药卫生—病原生物学]