Th17细胞在NOD小鼠糖尿病发病过程中的作用机制的研究  

The role of Th17 cells in NOD mice diabetes

作　　者：赵崴[1] 徐晓莹[2] 史艳华[2] 张小玲[2] 孔庆飞[3] 王广友[3] 孙博[3] 

机构地区：[1]哈尔滨医科大学细胞生物学教研室,150081 [2]哈尔滨医科大学生物技术教学实验中心 [3]哈尔滨医科大学神经生物学教研室,150081

出　　处：《国际免疫学杂志》2013年第2期158-161,共4页International Journal of Immunology

基　　金：黑龙江省教育厅科学技术研究面上项目（12521211）

摘　　要：目的探讨Th17细胞在NOD小鼠糖尿病发生过程中的作用机制。方法取胰岛炎组（血糖浓度〈150mg／d1）、糖尿病组（血糖浓度250—400mg／d1）NOD小鼠的胰腺，利用组织病理学方法观察胰岛损伤及单核细胞的浸润情况，观察IL一17的表达情况；取不同血糖浓度NOD小鼠淋巴结细胞，利用流式细胞术检测Th1／Th2／Th17／调节性T细胞（Treg）细胞分布情况。结果随着血糖浓度的增加，Treg细胞数量下降，Th17细胞数量增加，表明Th17细胞参与了1型糖尿病的发展过程，NOD小鼠发病与Th17细胞数量增加有关。结论Th17细胞参与NOD小鼠1型糖尿病的发生、发展过程，是促进疾病发展的因素之一。Objective To study the role of Thl7 cells in the development of diabetes. Methods NOD mice were divided into different groups according to their blood glucose concentrations. IL-17 expression and mononuclear cell infiltration were obsered immnnohistochemistry methods. Thl/Th2/Thl7/Treg cell distri- bution was determined by flow cytometry. Results Along with the increasing concentration of glucose, the number of CIM ＋ Foxp3 ＋ T cells decreased, but the number of Th17 cells increased. Conclusion Th17 cells are involved in the development of type I diabetes and are one of the promoting factors for the diabetes.

关 键 词：NOD小鼠 TH17细胞 1型糖尿病 

分 类 号：R587.1[医药卫生—内分泌]