SSR引物及多态性位点数对陆地棉野生种系聚类结果的影响  

Effection of the Quantity of SSR Primer and Allele on Cluster Analysis of Gossypium hirsutum Linn Races

作　　者：高伟[1] 王坤波[1] 刘方[1] 王春英[1] 张香娣[1] 王玉红[1] 黎绍惠[1] 

机构地区：[1]中国农业科学院棉花研究所/棉花生物学国家重点实验室,安阳455000

出　　处：《植物遗传资源学报》2013年第2期237-242,共6页Journal of Plant Genetic Resources

基　　金：中央级公益性院所基本科研业务费资助项目(SJA0901;SJA1001)

摘　　要：利用SSR标记分析陆地棉野生种系的遗传多样性,对材料间相似系数的变异系数进行显著性测验和矩阵相关性测验,探讨引物和多态性位点数对研究结果准确性的影响。90对多态性引物在42份供试材料间共检测出530个等位位点,其中多态性位点440个,占83.01%。多态信息含量范围为0.046～0.888,平均为0.649;Shannon多样性指数在0.113～2.289之间变动,平均为1.248。显著性测验显示,当引物按PIC值降序排列时,利用25对引物或者150个多态性位点即可获得较准确的结果;升序排列时,至少需要50对引物或200个多态性位点才能获得较准确的结果。矩阵相关性测验显示,降序时20对、升序时50对引物或者达到150个多态性位点聚类即可达到90对引物时的精度。此外,在引物量较少时,扩增位点数较多的引物所提供的信息量更大,随着引物量的增加,这种差距趋于不明显;等位位点总数较少时,引物数量更重要,随着位点数的增加,引物信息含量的重要性已高于引物数起主导作用。综上,若要客观反映出42份陆地棉野生种系的遗传关系,有必要选用多态性引物30对,扩增多态性位点150个以上,增加引物到50对以上为佳。In order to study the influence between the number of markers applied or corresponding polymor- phic alleles investigated and the reliability of evaluation the genetic diversity of G. hirsutum L. races using SSR markers, significance tests were done base on coefficient of variation（CV） about genetic similarity coefficient, the correlation among cophenetic matrices were also examined using Mantel correlation test. A total of 90 pairs of SSR primers were used to amplify the 42 accessions of germplasm. 440 polymorphic bands were obtained, which ac- counting for 88.5% of the total 530 bands. For 90 primers, the polymorphism information content （PIC） was from 0. 046 to 0. 888, with the average of 0. 649. The Shannon-Weaver diversity index （H＇） ranged from 0.113 to 2. 289 and averaged at 1. 248. Significance test showed that relatively accurate results could be got by 25 primers or 150 polymorphic alleles when the primers were sorted based on PIC in descending order and by at least 50 primers or 200 polymorphic alleles in ascending sort order. The correlation among cophenetic matrices was also examined u- sing Mantel correlation test. The objectively genetic relationships could be reflected with 20 primers in descending sort order or 50 primers in ascending sort order or all 150 alleles. In addition, the primer offered more information which provided more alleles when less primer was used relatively, but the information of each primer provided was becoming not obvious with the adding of primers. When using less allele, the quantity of primer was more impor-tant, and the information was more important than quantity of primer as the alleles increase. In general, it＇ s neces- sary to use 30 pairs of primers and amplify 150 alleles, the objectively genetic relationships of 42 accessions of G. hirsutum L. races could be reflected, using more than 50 is better.

关 键 词：陆地棉 野生种系 SSR 引物 多态性位点 聚类 

分 类 号：S562[农业科学—作物学]