玉米雄穗颜色的QTL分析  

Identification of QTL for Tassel Color in Maize

作　　者：袁文娟[1] 覃鸿妮[1,2] 王旭[1] 王国强[1] 王久光[1] 刘志斋[1] 蔡一林[1] 

机构地区：[1]西南大学玉米研究所,北碚400715 [2]苏州工业园区服务外包职业学院,苏州215123

出　　处：《植物遗传资源学报》2013年第2期289-297,共9页Journal of Plant Genetic Resources

基　　金：重庆市重大科技专项(cstc2012gg80003;cstc201280006)

摘　　要：雄穗是玉米的重要生殖器官,不同品种间玉米的雄穗外观差异明显。对玉米雄穗的颜色进行遗传分析和QTL定位,筛选与雄穗颜色紧密连锁的分子标记,可以作为玉米的品种保护和品种鉴别的有用工具。同时,紫色雄穗中花色苷类色素含量较高,与玉米雄穗的抗虫性密切相关。本研究利用一个黑玉米自交系SDM为共同父本,分别与白玉米自交系木6和黄玉米自交系Mo17杂交,构建2个相关F2∶3群体,分别命名为MuS(木6×SDM)和MoS(Mo17×SDM),在云南和重庆两个不同的环境中种植,对玉米花药颜色(COAn)和花药护颖颜色(COCa)2个性状进行QTL定位。结果表明:玉米花药和花药护颖的颜色均为数量性状,受主效基因和微效基因共同控制。2个群体在2个环境中共检测到7个与花药颜色相关的QTL,位于第2、3、6和10染色体上,其中位于第10染色体标记区间umc1196a-IDP8526内的QTL在重庆和云南同时表达,对表型的贡献率分别为23.17%和19.98%;2个群体在2个环境中共检测到9个与花药护颖颜色相关的QTL,位于第3、6、9和10染色体上,其中3个QTL为环境钝感QTL(在2个环境中均表达,且至少在1个环境中贡献率大于10%),分别位于第6染色体标记区间umc1979-umc1796、mmc0523-umc2006内和第10染色体标记区间umc1196a-umc2043内,对表型的贡献率为10.69%～59.30%。2个群体检测到的主效QTL的位置和效应高度一致,且控制花药颜色和花药护颖颜色2个性状的主效QTL有连锁分布的现象,主要表现在bins 6.04处的标记mmc0523和bins 10.04处的标记IDP8526附近。位于第6和第10染色体上的在不同环境和遗传背景下稳定的QTL可以作为进一步精细定位的靶位点,也可以为玉米雄穗颜色的分子标记辅助选择提供有价值的参考。Tassel is an important reproductive organ of maize and its appearance varies greatly in different maize varieties. Molecular markers tightly linked with tassel color could be screened by genetic analysis and QTL mapping on the color of the maize tassel. These molecular markers can be used as an useful tool for maize variety protection and species identification. Mean while, higher pigment content of anthocyanin in purple tassel is closely related to the insect resistance of maize tassel. In this paper,two related F2：3 populations derived from crosses of Mu6 x SDM（MuS） and Mo17 x SDM（ MoS）were used to identify QTLs for for two traits,i, e. ,the anther color（COAn） and the anther capes color（COCa） ,and the two populations were planted in two different environments in Yunnan and Chongqing for phenotyping. The results showed that the anther color and the anther cape color were both quanti- tative traits,jointly controlled by major genes and minor genes. Seven QTLs related to anther color were detected from the two populations in two different environments. They were located in chromosomes 2,3,6,and 10. The QTL within marker interval umc1196a-IDP8526 on chromosome 10 was both expressed in Chongqing and Yunnan, and contributed 23.17% and 19.98% of the phenotypic variation,respectively. Nine QTLs related to anther cape color were detected from the two groups in two environments,located in chromosome 3,6,9, and 10. Among them, three QTLs were stable between environments（with contribution rate more than 10% in at least one environment,detectedin two environments）. The three stable QTLs were located in the marker interval umc1979-umc1796 and mmc0523- umc2006 on chromosome 6 and the marker interval umc1196a-umc2043 on chromosome 10, explaining 10.69% to 59.30% of the phenotypic variation. The number,distribution, and effects of QTLs in two populations were highly consistent, and the major QTLs controlling the two traits of anther color and anther cape color co-localized with each other for both populat

关 键 词：玉米 花药颜色 花药护颖颜色 QTL分析 

分 类 号：S513[农业科学—作物学]