生物膜形成菌感染局部细胞因子的变化  

Change of cytokines in position infected by film-forming bacteria

作　　者：王国戗[1] 娄婷叶[1] 裴利宏[1] 

机构地区：[1]新乡医学院第一附属医院检验科,河南卫辉453100

出　　处：《中华医院感染学杂志》2013年第5期969-971,共3页Chinese Journal of Nosocomiology

摘　　要：目的研究生物膜形成菌引起的肺部感染进而导致感染部位细胞因子的变化以及变化规律,为临床治疗其感染治疗提供理疗依据。方法对临床上的支气管肺泡灌洗液标本培养出的菌株进行生物膜形成能力检测,将生物膜形成能力分为弱、中、强3档;对培养出上述细菌的支气管肺泡灌洗液进行白介素-4和r-干扰素细胞因子的检测,并同时检测患者血清中白介素-4和r-干扰素的水平。结果从支气管肺泡灌洗液中分离出的68株铜绿假单胞菌、鲍氏不动杆菌、肺炎克雷伯菌中有24株菌具有强的生物膜形成能力,21株生物膜形成能力较差,感染24株强生物膜形成能力菌的患者支气管肺泡灌洗液中的白介素-4水平(28.85±7.67)pg/ml显著高于感染21株弱生物膜形成能力菌的患者支气管肺泡灌洗液中白介素-4水平(20.75±7.63pg/ml)(P<0.05),感染强生物膜形成菌和弱生物膜形成菌的患者支气管肺泡灌洗液中的r-干扰素水平分别为(12.78±3.75)pg/ml和(14.01±3.63)pg/ml,两者间差异无统计学意义;感染24株强生物膜形成能力菌和21株弱生物膜形成能力菌的患者血清中的白介素-4水平分别为(18.06±7.12)pg/ml和(20.74±6.45)pg/ml,两者间差异无统计学意义,r-干扰素水平分别为(13.84±6.26)pg/ml和(15.14±5.77)pg/ml,两者间差异无统计学意义;68份患者支气管肺泡灌洗液中的白介素-4与血清中白介素-4检测结果分别为(27.43±12.82)pg/ml和(20.45±7.18)pg/ml,两者比较相关系数r=-0.146,P>0.05,r-干扰素水平分别为(13.41±4.09)pg/ml和(15.107±5.80)pg/ml,两者比较相关系数r=-0.103,P>0.05。结论强生物膜形成菌感染局部产生的白介素-4水平显著高于弱生物膜形成菌感染局部产生的白介素-4水平,而r-干扰素水平无显著的差异,这种细胞因子差异可为临床治疗肺部因该类细菌感染,特别是强生物膜形成菌造成肺部感染的治疗提供理论依据。OBJECTIVE To study the change of cytokines and the change rules aroused by the pulmonary infections of infection sites due to the formation of biofilm.METHODS The power of the biofilm formation was determined for the strains cultured from the bronchoalveolar lavage fluid specimens then were divided into the strong,medium and week group.The IL-4 and r-IFN level were detected in the bronchoalveolar lavage fluid and serum of patients,then the detection results were analyzed by SPSS 17.0 statistics software.RESULTS There were 24 strains with strong film-forming ability and 21 strains with weak film-forming ability among the 68 strains of Pseudomonas aeruginosa,Acinetobacter baumanii,and Klebsiella pneumoniae isolated from the bronchoalveolar lavage fluid.The IL-4 level（28.85±7.67pg/ml）in bronchoalveolar lavage fluid from patients infected by strong film-forming bacteria was significantly higher than（20.75±7.63 pg/ml） from the patients infected by weak biofilm-forming ability（P0.05）,but the levels of r-IFN in the bronchoalveolar lavage fluid from the patients infected by the strong film-forming bacteria and the patients infected by the weak film-forming bacteria were（12.78±3.75）pg/ml and（14.01±3.63）pg/ml,respectively,the difference was not statistically significant.The serum IL-4 levels of the patients infected by 24 strains of strong film-forming bacteria and the patients infected by 21 strains of film-forming bacteria were（18.06±7.12） pg/ml and（20.74±6.45） pg/ml,respectively,the difference was not statistically significant,the serum r-INF levels were（13.84±6.26） pg/ml and（15.14±5.77） pg/ml,respectively,the difference was not statistically significant.The IL-4 level of the bronchoalveolar lavage fluid and the serum IL-4 of the 68 patients were（27.43±12.82） pg/ml and（20.45±7.18） pg/ml,respectively,with the correlation coefficient of r=-0.146,（P0.05）,while the r-IFN levels were（13.41±4.09）pg/ml and（15.107±5.8 0）pg/ml,respectively,with the correlation c

关 键 词：生物被膜形成菌 支气管肺泡灌洗液 细胞因子 白介素-4 R-干扰素 

分 类 号：R378[医药卫生—病原生物学]