诱导小鼠胚胎干细胞在体外分化为骨骼肌细胞的实验研究  

Research of Inducing Mouse Embryonic Stem Cells Differentiate into Skeletal Muscle Cells in vitro

作　　者：叶枫[1] 佟慧丽[1] 杜巍[1] 严云勤[1] 

机构地区：[1]东北农业大学生命科学学院,哈尔滨150030

出　　处：《中国细胞生物学学报》2013年第3期273-281,共9页Chinese Journal of Cell Biology

基　　金：转基因生物新品种培育科技重大专项(批准号:2008ZX08007-002)资助的课题~~

摘　　要：小鼠胚胎干细胞是从胚泡未分化的内部细胞团中得到的干细胞,它在体外培养的环境中具有无限增殖、自我更新以及多向分化的特性。将小鼠胚胎干细胞在体外诱导分化为肌肉细胞,并且利用这些分化得来的肌肉细胞治疗肌肉退行性疾病,是干细胞研究领域的热点。该实验的目的在于筛选小鼠胚胎干细胞向骨骼肌细胞定向分化的实验条件,有效地将体外单层贴壁培养的小鼠胚胎干细胞诱导分化成骨骼肌细胞。最终发现,10-8mol/L维甲酸(retinoid acid,RA)+0.5%二甲基亚砜(dimethyl sulfoxide,DMSO)组诱导小鼠胚胎干细胞在体外分化成骨骼肌前体细胞的效率最高,分化得到的骨骼肌前体细胞经进一步纯化,能分化为多核的肌管。该实验为治疗肌肉退行性疾病提供了细胞来源,也为研究小鼠胚胎干细胞分化为骨骼肌细胞的机制提供了有利的条件。Mouse embryonic stem cells are separated from the inner cell mass of embryo, with the abilities of unlimited growth, self-renewal and differentiation potential when cultured in vitro. Mouse embryonic stem cells can be induced to differentiate into muscle cells, which can be used in treatment of muscle degenerative diseases and is becoming a research focus of the field. The aim of this study was to obtain the effective conditions of inducing mouse embryonic stem cells differentiate into skeletal muscle cells. Results showed that 10-8 mol/L retinoid acid （RA） and 0.5% dimethyl sulfoxide （DMSO） could induce mouse embryonic stem cells differentiate into skeletal muscle precur- sor cells with the highest efficiency, and the skeletal muscle precursor cells purified to differentiate into multinucleated myotubes. It provided cells for the treatment of muscle degenerative diseases and also can be beneficial to the study of the mechanism about mouse embryonic stem cells differentiate into skeletal muscle.

关 键 词：小鼠胚胎干细胞 骨骼肌细胞 分化 

分 类 号：Q813[生物学—生物工程]