壮药汗衣台配伍组方体外抗乙型肝炎病毒的疗效  

In vitro anti-HBV effect of polyherbal medicine of Chuang herb HanYiTai

作　　者：廖丹[1] 段雪琳[1] 

机构地区：[1]广西中医药大学基础医学院生理教研室,广西壮族自治区南宁市530001

出　　处：《世界华人消化杂志》2013年第7期614-618,共5页World Chinese Journal of Digestology

基　　金：广西自然科学基金资助项目;No.2010GXNSFA013198;广西中医药管理局中医药科技专项课题基金资助项目;No.GZKZ09-11;广西教育厅科研基金资助项目;No.200810LX002~~

摘　　要：目的:检测乙型肝炎病毒(hepatitis B virus,HBV)抗原和HBV DNA,比较壮药汗衣台的复方制剂与单味药在抗乙型肝炎病毒方面的疗效.方法:根据壮族民间常用的经验组方,不同浓度汗衣台及其配伍药分别与体外培养的2215细胞共同孵育,以XTT方法检测他们对2215细胞的毒性作用,选择各种药物对细胞无明显毒害的浓度进行组配;3d更换一次含药培养液,9d后收集上清液;用ELISA法测定上清液中乙型肝炎表面抗原(hepatitis B surface antigen,HBsAg)和乙型肝炎e抗原(hepatitis B e antigen,HBeAg)含量,荧光定量PCR法检测上清液中HBV-DNA的分泌.结果:复方组对HBsAg抗原的抑制作用稍高于单药组,但统计学上无显著意义;中低浓度的复方制剂F3-F5抑制HBeAg以及HBV-DNA分泌的作用明显强于相对应浓度的汗衣台单药组(P=0.002-0.009;P=0.038-0.05),而且抑制HBV-DNA分泌的疗效明显优于高浓度的复方制剂F1-F2(P=0.048-0.015).结论:组方配伍后,中低浓度的复方制剂抗病毒的综合疗效优于相对应浓度汗衣台单味药及高浓度复方制剂,各种药物成分相互影响、协同作用,不仅可以降低单个药物浓度,而且可以产生叠加增效作用.AIM： To compare the effect of polyherbal medi- cine and single herb of Chuang herb HanYiTai in resisting HBV by detecting HBV antigen and HBV DNA. METHODS： Different concentrations of HanY- iTai and other compounded herbs were co-incu- bated with 2215 cells in vitro, and their toxic ef- fects were detected by XTT method to determine the safe concentrations of HanYiTai and other herbs. Polyherbal compounds were matched with a variety of intoxic concentrations of herbs. Culture medium was changed once every 3 d,and supernatants were collected 9 d later. The contents of HBsAg and HBeAg in supernatants were measured using ELISA. Meanwhile, the secretion of HBV-DNA was detected using fluo- rescent quantitative PCR. RESULTS： The inhibitory effect of polyherbal medicine on the secretion of HBsAg antigen was slightly stronger than that of single HanYiTai group, but the difference was not statistically significant. The inhibitory effect of polyherbal medicine F3-F5 on the secretion of HBeAg and HBVDNA was significantly stronger than that of corresponding concentrations of single HanY- iTai （P = 0.002-0.009; P =0.038-0.05）. Polyherbal medicine at low and middle concentrations （F3-F5） showed stronger inhibitory effect on the secre- tion of HBVDNA than that at high concentra- tions （F1-F2） （P = 0.048-0.015）. CONCLUSION： Anti-HBV effect of low con- centration of polyherbal medicine was better than that of high concentration of polyherbal medicine and corresponding concentration of HanyiTai single herb. The mutual influence and synergy of various pharmaceutical ingredients can not only reduce the concentration of a single drug but also produce a superimposed synergis- tic effect.

关 键 词：汗衣台 复方中药制剂 2215细胞 乙型肝炎病毒 体外 

分 类 号：R512.62[医药卫生—内科学]