染料木黄酮对人结肠癌SW620细胞生长的抑制  

Genistein inhibits cell proliferation in human colon cancer cell line SW620

作　　者：梅庆步[1] 赵忠新 郑立红[1] 陈萍[1] 刘丹[1] 

机构地区：[1]齐齐哈尔医学院生物遗传教研室,黑龙江省齐齐哈尔市161006 [2]齐齐哈尔市建华医院普外科,黑龙江省齐齐哈尔市161006

出　　处：《世界华人消化杂志》2013年第7期619-623,共5页World Chinese Journal of Digestology

基　　金：齐齐哈尔市科技局基金资助项目~~

摘　　要：目的:探讨染料木黄酮对人结肠癌SW620细胞增殖的影响.方法:采用MTT法、Giemsa染色法及Hoechst 33258荧光染色法检测染料木黄酮对SW620细胞生长的影响及形态学变化.结果:在一定浓度范围内染料木黄酮可抑制人结肠癌SW620细胞的增殖,其抑制率与作用时间和药物剂量呈依赖关系;光镜下可见,药物组细胞数量明显减少,细胞膜边界不清,胞浆内可见空泡样结构,核膜不清晰,染色质边缘化,染色质分割成块状;荧光显微镜下可见,药物组部分细胞核呈现亮蓝色荧光的为凋亡细胞,细胞核呈波纹状改变,个别细胞核可见碎块状荧光信号,凋亡指数为27.18%.结论:染料木黄酮对人结肠癌SW620细胞的增殖有明显的抑制作用,诱导细胞凋亡可能是染料木黄酮抗结肠癌的作用机制之一.METHODS： MTT assay, Giemsa staining and Hoechst 33258 fluorescent staining were used to detect the effect of genistein on the growth and morphologic changes of SW620 cells. RESULTS： Genistein could effectively inhibit the proliferation of SW620 cells in a dose- and time-dependent manner. Under a light micro- scope, cells treated with genistein showed signif- icantly reduced number, unclear cell membraneand nuclear boundaries, chromatin marginal- ization, and chromatin segmentation. Under a fluorescence microscope, the nuclei of some cells treated with genistein presented light blue fluo- rescence and corrugated changes, which were characteristics of apoptotic cells. The nuclei of few cells presented fragmented fluorescence sig- nal. Apoptosis index was 27.18%. CONCLUSION： Genistein has an obvious in- hibitory effect on the proliferation of SW620 cells, and inducing apoptosis may be one of the mechanisms that are related to anticarcinogenic action of genistein.

关 键 词：染料木黄酮 SW620 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]