系统性红斑狼疮患者血清抗内皮细胞抗体和抗β_2糖蛋白I抗体水平及其对人脐静脉内皮细胞增殖的影响  

The level of anti-endothelial cell antibody and anti-β_2 GP-Ⅰantibody in serum with systemic lupus erythematosus and the variation of human umbilical veins endothelial cell cultured in serum with SLE

作　　者：张梦莹[1] 李志[2] 吕坤[1] 钟民[1] 所起凤[1] 钟正灵[3] 

机构地区：[1]皖南医学院弋矶山医院中心实验室 [2]皖南医学院弋矶山医院风湿科 [3]皖南医学院弋矶山医院临床药学部

出　　处：《中国临床药理学与治疗学》2013年第2期142-146,共5页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：皖南医学院中青年科研基金(WK2011F27)

摘　　要：目的:检测抗内皮细胞抗体(AECA)和抗β2GP-Ⅰ抗体在SLE患者血清中的水平及探讨AECA对人脐静脉内皮细胞(HUVEC)增殖的影响。方法:收集系统性红斑狼疮(SLE)患者90例和正常对照40例,留取血清,经酶联免疫吸附法(ELISA)检测AECA和抗β2GP-Ⅰ抗体水平。收集SLE患者部分临床指标,分析AECA和抗β2GP-Ⅰ抗体水平与临床指标间的关系。将不同浓度AECA的SLE患者血清加入HUVEC培养基,应用四唑盐比色试验(MTT)法检测各组OD值判断内皮细胞增殖状态。结果:SLE患者血清中AECA和抗β2GP-Ⅰ抗体水平显著高于正常对照组(P<0.01)。在90例SLE患者中33例抗β2GP-Ⅰ抗体阳性,该33例SLE患者对应的AE-CA、APTT水平和SLE活动评分(SLEDAI)积分均显著高于其余57例SLE患者(P<0.01,P<0.05,P<0.05)。同时该33例患者AECA和抗β2GP-Ⅰ抗体水平呈高度正相关(r=0.984,P<0.01),AECA和抗β2GP-Ⅰ抗体水平均与SLE-DAI呈正相关(P<0.05),抗β2GP-Ⅰ抗体水平与凝血酶原时间(PT)间存在正相关(r=0.349,P<0.05)。空白对照组、健康对照血清组和低浓度AECA血清组间OD值无差异,而高浓度AECA血清组OD值显著低于以上3组(P<0.05)。结论:AECA和抗β2GP-Ⅰ抗体与SLE疾病活动相关,AECA在体外对HUVEC增殖有抑制作用。AIM： To investigate the level of anti-endothelial cell antibody （AECA） and anti- β2GP-1 antibody in serum with systemic lupus erythematosus （SLE） and observe the variation of human umbilical veins endothelial cell （HU- VEC） cultured in serum with SLE. METHODS： The level of AECA and anti-β2GP-1antibody were detected by enzyme linked immunosorbent assay（ELISA） in serum with SLE and 40 health- y persons. Besides, the systemic lupus erythe- matosus disease activity index （SLEDAI） were collected for analyzing the relationship of the level of AECA and anti-β2GP- 1 antibody and it. The HUVEC were cultured with the serum in SLE, and the influence of it were observed by methyl thiazolyl tetrazolium（MTT）. RESULTS： The level of AECA and anti-β2GP- 1 antibody in serum with SLE were significantly higher than that of control. The anti-β2GP-1 antibody was positive in 33 cases among 90 patients with SLE. The level of AECA and anti-β2GP-antibody and SLE active index （SLEDAI） of 33 patients were respectively significant higher than that of 57 pa- tients （P〈0.01, P〈0.05, P〈0.05）. There was a significantly positive correlation between the level of AECA and anti-β2 GP-1 antibody （r =0. 984, P〈0.01）. There was a positive corre- lation between the level of AECA and anti-β2 GP- 1 antibody with SLEDAI （P〈0.05）. The level of anti-β2GP-1 antibody was significant correla- tion with the index of PT （r= 0.349, P 0.05）. The proliferation of endothelial cells was inhibited by the serum of SLE with high level of AECA. There was no difference among three groups, the blank control group and the healthy group and the group with low AECA. The OD of group with the serum high expression of AECA was significantly higher than that of other groups （P〈0.05）. CONCLUSION： The level of AECA and anti-β2GP- 1 antibody was correlative with the active of SLE. AECA can inhibit the proliferation of endothelial cell in vitro.

关 键 词：系统性红斑狼疮 抗内皮细胞抗体 抗β2糖蛋白Ⅰ抗体 人脐静脉内皮细胞 

分 类 号：R965.2[医药卫生—药理学]