马齿苋总黄酮对血小板源性生长因子致家兔血管平滑肌细胞增殖的抑制作用  

Effects of portulaca total flavone on proliferation of vascular smoothmuscle stimulated by PDGF-BB

作　　者：卢新华[1] 黄煌[1] 谭斌[1] 邓华菲[2] 王桂霞[1] 左克强[3] 李坚[2] 何军山[4] 

机构地区：[1]湘南学院基础医学部药理教研室 [2]湘南学院基础医学部病理生理教研室 [3]湘南学院基础医学部生物化学教研室 [4]湘南学院基础医学部预防医学系预防教研室

出　　处：《中国临床药理学与治疗学》2013年第2期156-160,共5页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：湖南省自然科学基金项目(06JJ50064);湖南省教育厅十二.五重点建设学科基金资助项目编号;湖南省心脑血管天然药物研究重点实验室基金项目资助

摘　　要：目的:研究马齿苋总黄酮(Portulaca totalflavone,PTF)对血小板源性生长因子-BB型(PDGF-BB)致血管平滑肌细胞(Vascularsmoothmuscle cell,VSMC)增殖的影响并探讨其机制。方法:采用组织贴壁法培养家兔胸主动脉血管平滑肌细胞,细胞计数法观察PTF(8、24、72mg/L)对PDGF-BB所致的VSMC增殖作用的影响;氚-胸腺嘧啶核苷(3H-TdR)掺入方法测定VSMC DNA的合成;流式细胞仪分析增殖细胞周期,荧光分光光度计测定VSMC内[Ca2+]i。结果:PTF以浓度依赖方式抑制PDGF-BB诱导的VSMC增殖、DNA合成,血管平滑肌细胞处于G1/G0期的细胞数增多,而G2/S期的细胞数显著减少(P<0.01);能抑制高K+诱导的VSMC内[Ca2+]i升高(P<0.01)。结论:PTF可明显抑制PDGF-BB诱导的VSMC增殖,其作用机制可能与其降低VSMC内高钙浓度[Ca2+]i有关。AIM. To study the effects of por- tulaca total flavone （PTF） on proliferation in- duced by PDGF-BB of rabbit aortic smooth mus- cle cells （VSMCs）and explore its mechanisms. METHODS. The VSMCs of cultured organiza- tion-adherence was induced to proliferate by PDGF-BB, and effects of PTF on VSMCs was tested by cell counting method and 3H-thymi- dine （3H-TdR） incorporation test; The effect of PTF on cell proliferative cycle of VSMC was ob- served by flow cytomytry technique; The intra- cellular calcium concentration was investigated by {luorimetry. RESULTS. PTF inhibited the proliferation of VSMCs induced by PDGF-BB in a dose dependent manner, and DNA symthesis, the cells numbers of G1/Go phase were increased and that of Gz/S phase were decreased markedly （P 〈 0.01 ）. CONCLUSION. Proliferation of VSMCs induced by PDGF-BB can be inhibited by PTF. The mechanisms may be related with di- minished VSMCs [Ca2＋ ]i.

关 键 词：马齿苋总黄酮 血管平滑肌细胞 细胞增殖 血小板源性生长因子-BB型 氚-胸腺嘧啶核苷 细胞周期 细胞内钙 

分 类 号：R965.2[医药卫生—药理学]