温胆汤含药血清对PC12细胞Bax、Bcl-2mRNA表达的影响  

Effect of Bax/Bcl-2mRNA Expression of Wendan Decoction Medicated Serum on PC12 Cells

作　　者：付艳丽[1] 朱金华[1] 杨翠萍[1] 万红娇[1] 

机构地区：[1]江西中医学院,南昌330004

出　　处：《江西中医学院学报》2012年第4期51-54,共4页Journal of Jiangxi College of Traditional Chinese Medicine

基　　金：江西省自然科学基金项目(项目编号:2010GZY0162);江西省卫生厅中医药重点项目(项目编号:2010Z02)

摘　　要：目的:探讨温胆汤含药血清对PC12细胞Bax/Bcl-2mRNA表达的影响。方法:(1)含药血清制备:30只SD大鼠随机分为三组:温胆汤高剂量组(A),温胆汤中剂量组(B),温胆汤低剂量组(C)。温胆汤灌胃,一天一次,8天后处死大鼠,股动脉取血,离心取血清,灭活无菌过滤备用。(2)PC12细胞分组培养:将PC12细胞分为4组,即:正常对照组(普通培养基培养)、温胆汤高剂量含药血清组、温胆汤中剂量含药血清组、温胆汤低剂量含药血清组,按照相应的分组更换含药血清进行培养16小时后,提取总mRNA,应用逆转录聚合酶链式反应(RT-PCR)法,检测PC12细胞Bax/Bcl-2mRNA的表达量。结果:温胆汤各组Bax mRNA的表达量较对照组有所降低,有显著性差异(P<0.05或P<0.01);而温胆汤高剂量组和温胆汤中剂量组使Bcl-2 mRNA的表达量比对照组有所升高,有显著性差异(P<0.05或P<0.01)。结论:温胆汤能够降低PC12细胞Bax mRNA的表达量,并且可升高Bcl-2 mRNA的表达量。Objective： To study the effect of Connexin Bax/Bcl-2mRNA expression of Wendan decoction medicated serum onPC12 cells.Methods： 1. Medicated serum preparation： 30 SD rats were divided three groups by random.They were Wendan Decoction high-dosage group （A）, Wendan Decoction middle-dosage group （B） and Wendan Decoction low-dosage group （ C） respective-ly.A, B and C groups were lavaged Wendan Decoction every day.After 8 days all rats were put to death. Then take blood from femoral artery, which centrifuge to take serum, and inactivate and sterilize them to set aside.2, PC12 cells group training： The PC12 cells will divided into 4 groups, they are comparison group （common culture media）, WenDan decoction high-dosage medicated serum group, WenDan decoction middle-dosage medicated serum group and WenDan decoction low-dosage medicated serum group.Accord- ing to the grouping of corresponding change medicated serum to train after 16 h, then extract total rnRNA .Using drug-containing serum to train PC12 cells after 16 hours, application RT-PCR half quantitative assay to test the expression of Bax mRNA and Bcl-2 mRNA by Wen Dan Decoction medicated serum for PC 12 cells. Results： the Bax mRNA expression of Wendan decoction Groups was lower than that of comparison group （P〈0.05 or P〈0.01） .And the Bcl-2 mRNA expression of Wendan Decoction high-dosagel group and Wendan Decoction middle-dosage group were rise compared with that of comparison group （P〈0.05 or P〈0.01 } .Conclusions： Wendan Decoction can reduce the expression of Bax mRNA and rise the expression of Bcl-2mRNA on the pC 12 cells.

关 键 词：含药血清 PC12 BAX BCL-2MRNA 表达 

分 类 号：R285.5[医药卫生—中药学]