酸枣仁及伪品滇枣仁的质量分析  

Species Differentiation and Quality Assessment of Ziziphus jujuba var.spinosa Based on the HPLC Fingerprint and Quantitative Analysis

作　　者：张翠英[1] 王阶[1] 郭丽丽 程惠平[1] 崔翰明[1] 吴萍[1] 董宇[1] 黄世敬[1] 

机构地区：[1]中国中医科学院广安门医院,北京100053

出　　处：《中药材》2012年第11期1758-1762,共5页Journal of Chinese Medicinal Materials

基　　金：国家重大新药创制科技重大专项(2009ZX09103-355)

摘　　要：目的:建立酸枣仁的多成分HPLC含量测定及HPLC指纹图谱分析方法,为鉴定酸枣仁的伪品滇枣仁提供可靠的科学方法。方法:采用Agilent TC-C18BDS(250 mm×4.6 mm,5μm)色谱柱;流动相为乙腈-水进行梯度洗脱;ELSD漂移管温度:110.5℃,气体流速:3.1 mL/min;流速:1.0 mL/min;柱温:30℃;进样量:20μL。结果:利用所建立的HPLC色谱分析方法,通过对27批商品酸枣仁中斯皮诺素、酸枣仁皂苷A、酸枣仁皂苷B和白桦脂酸4种活性成分的含量分析,并结合指纹图谱分析可以得出:正品酸枣仁与伪品滇枣仁在有效成分含量及指纹图谱方面具有明显的鉴别特征,易于鉴别。结论:本法专属性强、重现性好,可用于酸枣仁的质量控制及伪品滇枣仁的鉴别。Objective：To establish an HPLC method of a characteristical chemical fingerprint analysis in combination with simulta- neous determination of four bioactive components for species differentiation and quality assessment of Ziziphus jujuba var. spinosa. Meth- ods： The chromatographic separation was performed on an Agilent TC-C18 BDS （250 mm ×4. 6 mm, 5 μm） column. The mobile phase consisted of acetonitrile and water in a linear gradient elution procedure. The evaporator tube temperature of ELSD was set at 110. 5 ~C with the nebulizing gas flow rate of 3. 1 mL/min and the flow rate of mobile phase was 1.0 mL/min. The column was maintained at 30 ℃. The injection volume was 20 μL Results ： HPLC methodology for beth chemical fingerprint analysis and quantitative determina- tion of four active ingredients were validated, respectively. According to the contents of the four ingredients and the chemical finger- prints of Ziziphusjujuba var. spinosa using principal component analysis,Ziziphusjujuba var. spinosa was different from the fake derived from the seeds of Ziziphus mauritiana. Conclusion： The developed HPLC method is exclusive and repetitive for the species identifica- tion and quality evaluation of Ziziphus jujuba var. spinosa.

关 键 词：酸枣仁 滇枣仁 HPLC-ELSD 指纹图谱 主成分分析 

分 类 号：R282.5[医药卫生—中药学]